Inheritance and interactions of incompatibility alleles in the tetraploid sour cherry

• Published in: Theoretical and applied genetics Vol. 112; no. 2; pp. 315 - 326
• Main Authors: Boskovic, R.I, Wolfram, B, Tobutt, K.R, Cerovic, R, Sonneveld, T
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 2006; Berlin Springer Nature B.V
• Subjects: Alleles; Amino Acid Sequence; amino acid sequences; Biological and medical sciences; cherries; Chromosome Segregation; Classical genetics, quantitative genetics, hybrids; Cloning, Molecular; Crosses, Genetic; Fundamental and applied biological sciences. Psychology; Genetics of eukaryotes. Biological and molecular evolution; inheritance (genetics); Inheritance Patterns - genetics; Introns - genetics; Molecular Sequence Data; nucleotide sequences; plant fertility; pollen; pollen tube growth; pollen tubes; Polyploidy; Prunus - genetics; Prunus cerasus; Pteridophyta, spermatophyta; ribonucleases; Ribonucleases - metabolism; S-RNase; selfing; Sequence Analysis, DNA; styles; Vegetals; Incompatibility; Enzyme; Rosaceae; Esterases; Sour cherry; Prunus cerasus; Allele; Dicotyledones; S-RNases; Angiospermae; Hydrolases; Genetics; Spermatophyta; Agriculture; Inheritance(genetics); Pancreatic ribonuclease
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s00122-005-0130-0

Three progenies of sour cherry (Prunus cerasus) were analysed to correlate self-(in)compatibility status with S-RNase phenotype in this allotetraploid hybrid of sweet and ground cherry. Self-(in)compatibility was assessed in the field and by monitoring pollen tube growth after selfing. The S-RNase phenotypes were determined by isoelectric focusing of stylar proteins and staining for RNase activity and, for the parents, confirmed by PCR. Seedling phenotypes were generally consistent with disomic segregation of S-RNase alleles. The genetic arrangements of the parents were deduced to be 'Köröser' (self-incompatible) S ₁ S ₄ .S B S D , 'Schattenmorelle' (self-compatible) S ₆ S ₁₃ .S B S B , and clone 43.87 (self-compatible) S ₄ S ₁₃ .S B S B , where “.” separates the two homoeologous genomes. The presence of S ₄ and S ₆ alleles at the same locus led to self-incompatibility, whereas S ₁₃ and S B at homoeologous loci led to self-compatibility. The failure of certain heteroallelic genotypes in the three crosses or in the self-incompatible seedlings indicates that S ₄ and S ₆ are dominant to S B . However, the success of S ₁₃ S B pollen on styles expressing corresponding S-RNases indicates competitive interaction or lack of pollen-S components. In general, the universal compatibility of S ₁₃ S B pollen may explain the frequent occurrence of S ₁₃ and S B together in sour cherry cultivars. Alleles S B and S D , that are presumed to derive from ground cherry, and S ₁₃ , presumably from sweet cherry, were sequenced. Our findings contribute to an understanding of inheritance of self-(in)compatibility, facilitate screening of progenies for self-compatibility and provide a basis for studying molecular interactions in heteroallelic pollen.