Cell cycle dynamics and complement expression distinguishes mature haematopoietic subsets arising from hemogenic endothelium

• Published in: Cell cycle (Georgetown, Tex.) Vol. 16; no. 19; pp. 1835 - 1847
• Main Authors: Zape, Joan P, Lizama, Carlos O, Cautivo, Kelly M, Zovein, Ann C
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 02.10.2017
• Subjects: Animals; Cell Cycle - genetics; Cell Differentiation; Cell Division; Complement System Proteins - genetics; Complement System Proteins - metabolism; Embryo, Mammalian; Endothelium, Vascular - cytology; Endothelium, Vascular - growth & development; Endothelium, Vascular - metabolism; Female; Flow Cytometry; Gene Expression Regulation; Hemangioblasts - cytology; Hemangioblasts - metabolism; Hematopoiesis - genetics; Hematopoietic Stem Cells - cytology; Hematopoietic Stem Cells - metabolism; Mice; Mice, Transgenic; Pregnancy; Signal Transduction; Staining and Labeling - methods; hematopoietic stem cells; hematopoiesis; hemogenic endothelium
• ISSN: 1538-4101; 1551-4005
• DOI: 10.1080/15384101.2017.1361569

The emergence of haematopoietic stem and progenitor cells (HSPCs) from hemogenic endothelium results in the formation of sizeable HSPC clusters attached to the vascular wall. We evaluate the cell cycle and proliferation of HSPCs involved in cluster formation, as well as the molecular signatures from their initial appearance to the point when cluster cells are capable of adult engraftment (definitive HSCs). We uncover a non-clonal origin of HSPC clusters with differing cell cycle, migration, and cell signaling attributes. In addition, we find that the complement cascade is highly enriched in mature HSPC clusters, possibly delineating a new role for this pathway in engraftment.