Double intratibial injection of human tonsil-derived mesenchymal stromal cells recovers postmenopausal osteoporotic bone mass

• Published in: Cytotherapy (Oxford, England) Vol. 20; no. 8; pp. 1013 - 1027
• Main Authors: KIM, GYUNGAH, JIN, YOON MI, YU, YEONSIL, KIM, HA YEONG, JO, SANGMEE AHN, PARK, YOON JEONG, PARK, YOON SHIN, JO, INHO
• Format: Journal Article
• Language: English
• Published: England Elsevier Inc 01.08.2018
• Subjects: Advanced Basic Science; Animals; Bone Density - physiology; Child; Female; femoral head; Heterografts; Humans; Infusions, Intraosseous - methods; Injections; intratibial injection; Mesenchymal Stem Cell Transplantation - methods; Mesenchymal Stem Cells - cytology; Mice; Mice, Inbred ICR; Middle Aged; osteoporosis; Osteoporosis - diagnosis; Osteoporosis - pathology; Osteoporosis - therapy; Other; ovariectomized; Palatine Tonsil - cytology; Postmenopause - physiology; Remission Induction; Tibia - diagnostic imaging; tonsil-derived mesenchymal stem cells; visceral fat; femoral head; intratibial injection; ovariectomized; osteoporosis; tonsil-derived mesenchymal stem cells; visceral fat
• ISSN: 1465-3249; 1477-2566; 1477-2566
• DOI: 10.1016/j.jcyt.2018.06.008

Abstract Background and aims Osteoporosis, which is a disease characterized by weakening of the bone, affects a large portion of the senior population. The current therapeutic options for osteoporosis have side effects, and there is no effective treatment for severe osteoporosis. Thus, we urgently need new treatment strategies, such as topical therapies and/or safe and effective stem cell therapies. Methods We investigated the therapeutic potential of directly injecting human tonsil-derived mesenchymal stem cells (TMSC) into the right proximal tibias of ovariectomized postmenopausal osteoporosis model mice. Injections were given once (1×) or twice (2×) during the 3-month experimental period. At the end of the experiment, micro-computed tomographic images revealed some improvement in the proximal tibias and more significant improvement in the femoral heads of treated mice. Results Osteogenic effect was qualitatively and quantitatively more pronounced in TMSC/2×-treated mice. Furthermore, TMSC/2× mice exhibited significant recovery of the serum osteocalcin level, which is pathologically elevated in osteoporosis, and increased serum alkaline phosphatase, which indicates bone formation. TMSC therapy was generally well tolerated and caused no apparent toxicity in the experimental mice. Moreover, TMSC therapy reduced visceral fat. Conclusion Our results demonstrate that double injection of TMSC directly into the proximal tibia triggers recovery of osteoporosis, and thus could be a potential therapeutic approach for severe bone loss.