Contact angle changes induced by immunocomplex formation

• Published in: Analyst (London) Vol. 139; no. 6; pp. 1340 - 1344
• Main Authors: Kim, Jong-Hoon, Shen, Amy Q, Lee, Kyong-Hoon, Cangelosi, Gerard A, Chung, Jae-Hyun
• Format: Journal Article
• Language: English
• Published: England 21.03.2014
• Subjects: Affinity; Animals; Antibodies; Antibodies, Immobilized - chemistry; Antigen-Antibody Complex - immunology; Antigens; Bacteria; Binding; Capillarity; Cattle; Equipment Design; Escherichia coli - immunology; Escherichia coli - isolation & purification; Escherichia coli Infections - microbiology; Immunoassay; Immunoassay - instrumentation; Mycobacterium bovis - immunology; Mycobacterium bovis - isolation & purification; Tuberculosis, Bovine - microbiology

Immunoassays analyzing interactions between antigens and antibodies can be affected by capillary action together with binding affinity. This paper studies contact-angle changes of bacterial suspensions on antibody immobilized surfaces. The capillary action and the dried pattern of the bacterial susp...