Contact angle changes induced by immunocomplex formation

Immunoassays analyzing interactions between antigens and antibodies can be affected by capillary action together with binding affinity. This paper studies contact-angle changes of bacterial suspensions on antibody immobilized surfaces. The capillary action and the dried pattern of the bacterial susp...

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Bibliographic Details
Published inAnalyst (London) Vol. 139; no. 6; pp. 1340 - 1344
Main Authors Kim, Jong-Hoon, Shen, Amy Q, Lee, Kyong-Hoon, Cangelosi, Gerard A, Chung, Jae-Hyun
Format Journal Article
LanguageEnglish
Published England 21.03.2014
Subjects
Affinity
Animals
Antibodies
Antibodies, Immobilized - chemistry
Antigen-Antibody Complex - immunology
Antigens
Bacteria
Binding
Capillarity
Cattle
Equipment Design
Escherichia coli - immunology
Escherichia coli - isolation & purification
Escherichia coli Infections - microbiology
Immunoassay
Immunoassay - instrumentation
Mycobacterium bovis - immunology
Mycobacterium bovis - isolation & purification
Tuberculosis, Bovine - microbiology
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Summary:Immunoassays analyzing interactions between antigens and antibodies can be affected by capillary action together with binding affinity. This paper studies contact-angle changes of bacterial suspensions on antibody immobilized surfaces. The capillary action and the dried pattern of the bacterial suspensions are analyzed and correlated with specific- and nonspecific bindings between bacteria and antibodies.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0003-2654
1364-5528
DOI:10.1039/c3an02189k