Xplore® mRNA assays for the quantification of IL-1β and TNF-α mRNA in lipopolysaccharide-induced mouse macrophages

Because the accurate measurement of a number of cytokine mRNA transcripts provides valuable knowledge about cytokine gene regulation, we have developed the Xplore assay for the quantification of cytokine mRNA. This microplate-based assay is rapid (under four hours), quantitative over three orders of...

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Bibliographic Details
Published inBioTechniques Vol. 28; no. 6; pp. 1220 - 1225
Main Authors VAN ARSDELL, S. W, MURPHY, K. P, PAZMANY, C, ERICKSON, D, BURNS, C, MOODY, M. D
Format Journal Article
LanguageEnglish
Published Natick, MA Eaton 01.06.2000
Future Science Ltd
Subjects
Animals
Biological and medical sciences
Cell Line
Diverse techniques
Fundamental and applied biological sciences. Psychology
Interleukin-1 - genetics
Lipopolysaccharides - pharmacology
Macrophages - metabolism
Mice
Molecular and cellular biology
RNA, Messenger - analysis
Tumor Necrosis Factor-alpha - genetics
Rodentia
Cytokine
Interleukin 1β
Activation
Toxin
Vertebrata
Rapid technique
Mammalia
Analysis method
Messenger RNA
Mouse
Lipopolysaccharide
Tumor necrosis factor α
Quantitative analysis
Macrophage
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Summary:Because the accurate measurement of a number of cytokine mRNA transcripts provides valuable knowledge about cytokine gene regulation, we have developed the Xplore assay for the quantification of cytokine mRNA. This microplate-based assay is rapid (under four hours), quantitative over three orders of magnitude and carries no risk of false-positive values from contamination with amplified target. Here, we describe the use of Xplore assays to measure the steady-state mRNA levels of TNF-alpha and IL-1 beta produced by mouse WEHI and J774 macrophage-like cell lines.
Bibliography:ObjectType-Article-1
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ISSN:0736-6205
1940-9818
DOI:10.2144/00286pf01