Identification, structure and function of a novel tetraspaninhomologue from Spirometra erinaceieuropaei
To identify a full length c DNA sequence of a novel tetraspanin (TSP) homologue from Spirometra erinaceieuropaei and to predict the structure and function of its encoding protein using bioinformatics methods. Using the NCBI, EMBI, Expasy and other online sites, the open reading frame (ORF), conserve...
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Published in | Asian Pacific journal of tropical medicine Vol. 4; no. 9; pp. 739 - 742 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
India
Elsevier B.V
01.09.2011
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Subjects | |
Online Access | Get full text |
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Summary: | To identify a full length c DNA sequence of a novel tetraspanin (TSP) homologue from
Spirometra erinaceieuropaei and to predict the structure and function of its encoding protein using bioinformatics methods.
Using the NCBI, EMBI, Expasy and other online sites, the open reading frame (ORF), conserved domain, physical and chemical parameters, signal peptide, transmembrane domain, epitope, topological structures of the protein sequences were predicted. And Vector NTI software was used for multiple sequence alignment and phylogenetic tree construction.
The target sequence was 1132 bp length with a 681 bpbiggest ORF encoding 226 amino acids protein with typical TSP conserved domain. It was confirmed as full length c DNA of TSP16 from
Spirometra erinaceieuropaei and named as
SeTSP16 (GenBank accession number: JF728872). The predicted molecular weight and isoelectric point of the deduced protein were 24 750.5 Da and 7.88 Da, respectively. Compared with TSP16s from
Schistosoma japonicum and
Schistosoma mansoni, it showed similarity of 59% and 59%, respectively.
SeTSP16 contained four transmembrane domains (TM1-4), intracellular N and C-termini, one short small extracellular loop and one large extracellular loop. Four major epitopes that were significant different from the corresponding epitope regions of TSP16 from
Schistosoma mansoni and
Schistosoma japonicum were predicted.
The full length c DNA sequences of
SeTSP16 are identified. It encodes a transmembrane protein which might be an ideal diagnosis antigen and target molecule for antiparasitic drugs. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1995-7645 2352-4146 |
DOI: | 10.1016/S1995-7645(11)60184-3 |