Immunochemical evidence of homologies among 50 S ribosomal proteins of Bacillus stearothermophilus and Escherichia coli

Antibodies prepared against individual 50 S ribosomal subunit proteins from Escherichia coli were reacted with 70 S ribosomal proteins from Bacillus stearothermophilus in order to identify homologous protein pairs. B. stearothermophilus proteins were separated by two-dimensional polyacrylamide gel e...

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Bibliographic Details
Published inThe Journal of biological chemistry Vol. 256; no. 19; pp. 10111 - 10116
Main Authors Fahnestock, S R, Strycharz, W A, Marquis, D M
Format Journal Article
LanguageEnglish
Published United States American Society for Biochemistry and Molecular Biology 10.10.1981
Subjects
Antigen-Antibody Complex
Bacillus stearothermophilus
Electrophoresis, Polyacrylamide Gel
Escherichia coli
Escherichia coli - analysis
Geobacillus stearothermophilus - analysis
Immune Sera
Molecular Weight
Ribosomal Proteins - analysis
Ribosomal Proteins - immunology
Species Specificity
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Summary:Antibodies prepared against individual 50 S ribosomal subunit proteins from Escherichia coli were reacted with 70 S ribosomal proteins from Bacillus stearothermophilus in order to identify homologous protein pairs. B. stearothermophilus proteins were separated by two-dimensional polyacrylamide gel electrophoresis and transferred electrophoretically to diazobenzyloxymethyl paper to which they became covalently attached. The paper was then washed with antiserum followed by radioactive protein A, and the resulting antigen-antibody-protein A complexes were located by autoradiography. Seventeen cross-reacting protein pairs were identified.
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ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(19)68750-8