Sequence specific interstrand photocrosslinking for effective SNP typing

We describe a simple and inexpensive SNP typing method by using sequence specific interstrand photocrosslinking via p- carbamoylvinyl phenol nucleosides. Interstrand photocrosslinking showed a high degree of single nucleotide speci. city as high as 10(3)- fold and more, and can be used in the diagno...

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Bibliographic Details
Published inOrganic & biomolecular chemistry Vol. 5; no. 16; pp. 2583 - 2586
Main Authors Ami, Takehiro, Ito, Katsuya, Yoshimura, Yoshinaga, Fujimoto, Kenzo
Format Journal Article
LanguageEnglish
Published CAMBRIDGE Royal Soc Chemistry 01.01.2007
Subjects
Base Sequence
Chemistry
Chemistry, Organic
Cross-Linking Reagents - chemistry
Cross-Linking Reagents - radiation effects
DNA - chemistry
DNA - radiation effects
Nucleic Acid Conformation
Nucleosides - chemistry
Nucleosides - radiation effects
Oligodeoxyribonucleotides - chemistry
Oligonucleotide Array Sequence Analysis - methods
Phenols - chemistry
Phenols - radiation effects
Photochemistry
Physical Sciences
Polymorphism, Single Nucleotide - radiation effects
Science & Technology
Sequence Analysis, DNA - methods
Substrate Specificity
Ultraviolet Rays
POINT MUTATIONS
DNA PHOTOLIGATION
RNA
DISCRIMINATION
CHIPS
LIGATION
HYBRIDIZATION
SELECTIVITY
SINGLE NUCLEOTIDE POLYMORPHISMS
AUTOLIGATION
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Summary:We describe a simple and inexpensive SNP typing method by using sequence specific interstrand photocrosslinking via p- carbamoylvinyl phenol nucleosides. Interstrand photocrosslinking showed a high degree of single nucleotide speci. city as high as 10(3)- fold and more, and can be used in the diagnostic detection of DNA sequences.
ISSN:1477-0520
1477-0539
DOI:10.1039/b708264a