Potassium channel induction by the Ras/Raf signal transduction cascade
p21ras plays a critical role in cell growth, differentiation, and oncogenic transformation. However, the final physiological effectors of p21ras-mediated signal transduction remain to be determined. We have used patch clamp electrophysiology, pharmacological agents, and transfection with specific Ra...
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Published in | The Journal of biological chemistry Vol. 269; no. 49; pp. 31183 - 31189 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
United States
American Society for Biochemistry and Molecular Biology
09.12.1994
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Subjects | |
Online Access | Get full text |
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Summary: | p21ras plays a critical role in cell growth, differentiation, and oncogenic transformation. However, the final physiological
effectors of p21ras-mediated signal transduction remain to be determined. We have used patch clamp electrophysiology, pharmacological
agents, and transfection with specific Ras or Raf plasmids, to demonstrate that induction of a unique Ca(2+)-activated K+
channel in murine fibroblast cell lines depends on p21ras and its immediate downstream target, the Raf kinase. The importance
of this channel in mitogenic signaling is further indicated by its induction in nontransformed cells by epidermal growth factor
and platelet-derived growth factor and the ability of K+ channel blockers to inhibit cell proliferation. We suggest that this
Ca(2+)-activated K+ channel is one ultimate physiological target of p21ras-mediated signal transduction and that it may play
a role in cell proliferation and ras transformation. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 0021-9258 1083-351X |
DOI: | 10.1016/S0021-9258(18)47407-8 |