Phenolic amides from Tribulus terrestris and their inhibitory effects on nitric oxide production in RAW 264.7 cells

• Published in: Archives of pharmacal research Vol. 41; no. 2; pp. 192 - 195
• Main Authors: Kim, Hyung Sik, Lee, Jin Woo, Jang, Hari, Le, Thi Phuong Linh, Kim, Jun Gu, Lee, Moon Soon, Hong, Jin Tae, Lee, Mi Kyeong, Hwang, Bang Yeon
• Format: Journal Article
• Language: English
• Published: Seoul Pharmaceutical Society of Korea 01.02.2018; 대한약학회
• Subjects: amides; Amides - chemistry; Amides - isolation & purification; Amides - pharmacology; Animals; fruits; inhibitory concentration 50; mass spectrometry; Medicine; Mice; nitric oxide; Nitric Oxide - antagonists & inhibitors; Nitric Oxide - metabolism; nuclear magnetic resonance spectroscopy; Pharmacology/Toxicology; Pharmacy; Phenols - chemistry; Phenols - isolation & purification; Phenols - pharmacology; Plant Extracts - chemistry; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; RAW 264.7 Cells; Research Article; Tribulus; Tribulus terrestris; 약학; Zygophyllaceae; Phenolic amide, Nitric oxide production inhibitor; Tribulus terrestris
• ISSN: 0253-6269; 1976-3786; 1976-3786
• DOI: 10.1007/s12272-017-0984-0

A new phenolic amide, named cis -terrestriamide ( 7 ), together with ten known compounds ( 1 – 6, 8 – 11 ), were isolated from the methanolic extract of the fruits of Tribulus terrestris . The structure of 7 was elucidated on the basis of extensive analyses of 1D and 2D nuclear magnetic resonance spectroscopic and high resolution mass spectrometry data. Compounds 1 , 2 , 5 , 6 , 8 , 9 , and 11 exhibited inhibitory effects on the lipopolysaccharide-stimulated nitric oxide production in RAW 264.7 cells, with IC 50 values of 18.7–49.4 μM.