Investigation of the effect of SRSF9 overexpression on HIV-1 production

Serine-arginine-rich splicing factors (SRSFs) are members of RNA processing proteins in the serine-arginine-rich (SR) family that could regulate the alternative splicing of the human immunodeficiency virus-1 (HIV-1). Whether SRSF9 has any effect on HIV-1 regulation requires elucidation. Here, we rep...

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Bibliographic Details
Published inBMB reports Vol. 55; no. 12; pp. 639 - 644
Main Authors Kim, Ga-Na, Yu, Kyung-Lee, Kim, Hae-In, You, Ji Chang
Format Journal Article
LanguageEnglish
Published Korea (South) Korean Society for Biochemistry and Molecular Biology 31.12.2022
Subjects
Alternative Splicing - genetics
HEK293 Cells
HIV-1 - metabolism
Humans
RNA Splicing - genetics
RNA, Messenger - genetics
RNA, Messenger - metabolism
Serine-Arginine Splicing Factors - genetics
Serine-Arginine Splicing Factors - metabolism
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Summary:Serine-arginine-rich splicing factors (SRSFs) are members of RNA processing proteins in the serine-arginine-rich (SR) family that could regulate the alternative splicing of the human immunodeficiency virus-1 (HIV-1). Whether SRSF9 has any effect on HIV-1 regulation requires elucidation. Here, we report for the first time the effects and mechanisms of SRSF9 on HIV-1 regulation. The overexpression of SRSF9 inhibits viral production and infectivity in both HEK293T and MT-4 cells. Deletion analysis of SRSF9 determined that the RNA regulation motif domain of SRSF9 is important for anti-HIV-1 effects. Furthermore, overexpression of SRSF9 increases multiple spliced forms of viral mRNA, such as Vpr mRNA. These data suggest that SRSF9 overexpression inhibits HIV-1 production by inducing the imbalanced HIV-1 mRNA splicing that could be exploited further for a novel HIV-1 therapeutic molecule. [BMB Reports 2022; 55(12): 639-644].
ISSN:1976-6696
1976-670X
DOI:10.5483/BMBRep.2022.55.12.166