Replication Timing of Human Chromosome 6

• Published in: Cell cycle (Georgetown, Tex.) Vol. 4; no. 1; pp. 172 - 176
• Main Authors: Woodfine, Kathryn, Beare, David M., Ichimura, Koichi, Debernardi, Silvana, Mungall, Andrew J., Fiegler, Heike, Collins, V. Peter, Carter, Nigel P., Dunham, Ian
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 01.01.2005
• Subjects: Binding; Biology; Bioscience; Calcium; Cancer; Cell; Cell Line; Chromosome Mapping; Chromosomes, Human, Pair 6 - genetics; Chromosomes, Human, Pair 6 - physiology; Cycle; Cytosine - analysis; DNA - chemistry; DNA - genetics; DNA Repeat Expansion; DNA Replication Timing; Epigenesis, Genetic; G1 Phase - genetics; G1 Phase - physiology; Gene Expression Regulation; Guanine - analysis; Humans; Landes; Major Histocompatibility Complex - genetics; Oligonucleotide Array Sequence Analysis - methods; Organogenesis; Proteins; S Phase - genetics; S Phase - physiology; Transcription, Genetic
• ISSN: 1538-4101; 1551-4005
• DOI: 10.4161/cc.4.1.1350

Genomic microarrays have been used to assess DNA replication timing in a variety of eukaryotic organisms1-3. A replication timing map of the human genome has already been published at a 1Mb resolution3. Here we describe how the same method can be used to assess the replication timing of chromosome 6 with a greater resolution using an array of overlapping tile path clones. We report the replication timing map of the whole of chromosome 6 in general, and the MHC region in particular. Positive correlations are observed between replication timing and a number of genomic features including GC content, repeat content and transcriptional activity.