Activities of Hydrolases and Oxidases as Influenced by the Application of Monocrotophos in Sandy Loam Soil of Rajasthan

• Published in: Applied biochemistry and biotechnology Vol. 172; no. 7; pp. 3570 - 3582
• Main Authors: Jain, Rachna, Garg, Veena
• Format: Journal Article
• Language: English
• Published: Boston Springer-Verlag 01.04.2014; Springer US; Springer; Springer Nature B.V
• Subjects: acid phosphatase; additive effect; alkaline phosphatase; beta-fructofuranosidase; Biochemistry; Biological and medical sciences; Biotechnology; Chemistry; Chemistry and Materials Science; endo-1,4-beta-glucanase; Enzymatic activity; enzyme activity; Enzymes; fauna; Flora; Fundamental and applied biological sciences. Psychology; Hydrolases - analysis; India; Loam soils; monocrotophos; Monocrotophos - analysis; Oxidoreductases - analysis; Pesticides; Pesticides - analysis; proteinases; Sandy loam; sandy loam soils; Sandy soils; Soil - chemistry; Soil microorganisms; temperature; India; India, Rajasthan; Soil; Hydrolases; Enzyme activity; Pesticide; Enzymatic activity; Enzyme; Oxidase; Pesticides; Oxidoreductases; Sandy soil; Application
• ISSN: 0273-2289; 1559-0291
• DOI: 10.1007/s12010-014-0789-2

Continuous and repeated use of pesticides affects soil microbial flora and fauna and hence indirectly affects the activity of diverse microbial enzymes present within it. The present study investigates the interaction effect of different concentrations of monocrotophos on diverse hydrolases and oxidases, viz., protease, alkaline phosphatase, acid phosphatase, cellulase, amylase, invertase, arginine deaminase, and dehydrogenase, present in sandy loam soil of Rajasthan under in vitro conditions for 30 days. Soil sample was inoculated with three different concentrations of monocrotophos, viz., 50, 100, and 150 μg kg⁻¹, and incubated in dark at room temperature. At regular interval of 5 days, sample was withdrawn and enzyme activity was calculated and compared with that of control. Application of various concentrations of monocrotophos enhanced the activity of diverse enzymes present in soil. Therefore, the study revealed synergistic or additive effect of monocrotophos on all the tested microbial enzyme entities. Increasing concentration of the pesticide, however, poses an antagonistic interaction on the increment of different enzymes activities. Therefore, it can be concluded from the study that monocrotophos impose a positive effect at low concentration of pesticide, whereas high concentration poses negative effect on the activity of different enzymes present in soil.