Evaluating the Engineered Saccharomyces cerevisiae With High Spermidine Contents for Increased Tolerance to Lactic, Succinic, and Malic Acids and Increased Xylose Fermentation

• Published in: Biotechnology and bioprocess engineering Vol. 26; no. 1; pp. 47 - 54
• Main Authors: Kim, Sun-Ki, Auh, Joong-Hyuck
• Format: Journal Article
• Language: English
• Published: Seoul The Korean Society for Applied Biological Chemistry 01.02.2021; Springer Nature B.V; 한국생물공학회
• Subjects: Acids; biomass; bioprocessing; Biotechnology; carbon; Carbon sources; Chemistry; culture media; Dry cells; Ethanol; Fermentation; Fungi; glucose; Growth rate; Industrial and Production Engineering; Lignocellulose; Malic acid; Organic acids; Prokaryotes; prokaryotic cells; Research Paper; Saccharomyces cerevisiae; specific growth rate; Spermidine; Xylose; Yeast; 생물공학; organic acid tolerance; spermidine; xylose fermentation
• ISSN: 1226-8372; 1976-3816
• DOI: 10.1007/s12257-020-0020-y

Saccharomyces cerevisiae is a promising candidate for production of organic acids as it is more tolerant to these acids than the prokaryotes. However, the large-scale production of organic acids from lignocellulosic biomass is limited by their accumulation in the growth medium and inability of xylose fermentation by S. cerevisiae . Here we showed that high intracellular spermidine (SPD) contents confers enhanced tolerance to lactic, succinic, and malic acids in S. cerevisiae . Specifically, in the presence of 20 g/L malic acid, the maximum specific growth rate and dry cell weight of a S. cerevisiae with two fold higher SPD content were 40% and 36% higher than those of the control strain. When a xylose assimilation pathway was introduced into an engineered strain with high SPD content, the resulting S. cerevisiae strain exhibited 23∼47% higher xylose consumption rate and 6∼16% higher ethanol productivity than those of the control strain during the four times of repeated-batch fermentations using a mixture of glucose and xylose as carbon sources. These results suggest that the strain developed in this study would serve as a platform strain for production of organic acids.