AMPA receptor endocytosis in the amygdala is involved in the disrupted reconsolidation of Methamphetamine-associated contextual memory

• Published in: Neurobiology of learning and memory Vol. 103; pp. 72 - 81
• Main Authors: Yu, Yang-Jung, Chang, Chih-Hua, Gean, Po-Wu
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 01.07.2013; Elsevier; Elsevier BV
• Subjects: Addictive behaviors; Adult and adolescent clinical studies; AMPA receptor; Amygdala; Amygdala - drug effects; Amygdala - metabolism; Animals; Anisomycin; Anisomycin - pharmacology; Association Learning - drug effects; Association Learning - physiology; Behavioral psychophysiology; Biological and medical sciences; Conditioning, Operant - drug effects; Dopamine Uptake Inhibitors - pharmacology; Drug addiction; Drug-Seeking Behavior - drug effects; Drug-Seeking Behavior - physiology; Endocytosis; Endocytosis - drug effects; Endocytosis - physiology; Extinction, Psychological - drug effects; Fundamental and applied biological sciences. Psychology; Male; Medical sciences; Memory; Memory - drug effects; Memory - physiology; Methamphetamine; Methamphetamine - pharmacology; Mice; Neurons; Neuropharmacology; Pharmacology. Drug treatments; Protein Synthesis Inhibitors - pharmacology; Psychoanaleptics: cns stimulant, antidepressant agent, nootropic agent, mood stabilizer; Psychoanaleptics: cns stimulant, antidepressant agent, nootropic agent, mood stabilizer..., (alzheimer disease); Psychology. Psychoanalysis. Psychiatry; Psychology. Psychophysiology; Psychopathology. Psychiatry; Psychopharmacology; Receptors, AMPA - metabolism; Reconsolidation; Drug addiction; Reconsolidation; AMPA receptor; Anisomycin; Amygdala; Methamphetamine; Amphetamine derivatives; Context; CNS stimulant; Psychotropic; Central nervous system; Metamfetamine; Basal ganglion; Glutamate receptor; Sympathomimetic; Encephalon; Endocytosis; Addiction; Amygdaloid nucleus
• ISSN: 1074-7427; 1095-9564
• DOI: 10.1016/j.nlm.2013.04.004

•Methamphetamine (MeAM) induced conditioned place preference (CPP) and behavioral sensitization.•MeAM CPP increased surface expression of GluR1 and GluR2 subunits of AMPA receptor in the basolateral amygdala (BLA).•Anisomycin disrupted reconsolidation of MeAM CPP and prevented MeAM priming-induced reinstatement of CPP.•Intra-amygdala administration of Tat-GluR23Y prevented anisomycin disruption of MeAM memory reconsolidation. Repetitive drug taking induces neural long-lasting changes and results in compulsive drug-seeking behavior which may arise from enduring drug memory that impairs cognitive control of motivated behavior. Thus, disrupting these memories could reduce drug seeking. Here, we used a conditioned place preference (CPP) procedure in mice to examine the role of AMPA receptor endocytosis in the basolateral amygdala (BLA) in the disrupted reconsolidation of Methamphetamine (MeAM) memory. Conditioning MeAM (2mg/kg, i.p.) for 3days in mice markedly increased the time spent in the MeAM-paired compartment tested 24h after the last injection (CPP test), indicating that MeAM induced a significant rewarding effect. Mice then received anisomycin or vehicle within 1h after CPP test and CPP was re-assessed 24h after CPP test. Mice injected with vehicle exhibited CPP for the previously MeAM-paired chamber whereas mice injected with anisomycin did not. Anisomycin had no effect on the CPP when CPP test was omitted. In addition, anisomycin treatment prevented MeAM priming-induced reinstatement of CPP suggesting the disruption of MeAM memory reconsolidation. MeAM CPP increased surface expression of GluR1 and GluR2 subunits of AMPA receptor in the BLA. Bilateral injection of Tat-GluR23Y, a synthetic peptide that blocked AMPA receptor endocytosis, prevented disruption of MeAM memory reconsolidation. These results suggest that AMPA receptor endocytosis in the BLA is critical for the anisomycin-mediated disruption of reconsolidation of MeAM reward memory.