Interaction of lactic acid bacteria with the gut immune system

• Published in: European journal of clinical nutrition Vol. 56 Suppl 4; no. S4; pp. S21 - S26
• Main Authors: Perdigón, G, Maldonado Galdeano, C, Valdez, J C, Medici, M
• Format: Journal Article
• Language: English
• Published: England Nature Publishing Group 01.12.2002
• Subjects: Acids; Animals; Antibodies; Antibody response; Antigens; Bacteria; Clinical nutrition; Cytokines; Cytokines - immunology; Drinking water; Functional foods & nutraceuticals; Gram-positive bacteria; Immune response; Immune system; Immunoglobulin G; Immunology; Interferon-gamma - immunology; Interleukin 10; Interleukin 12; Interleukin 2; Interleukin 4; Intestine, Small - immunology; Intestine, Small - microbiology; Lactic acid; Lactic acid bacteria; Lactobacilli; Lactobacillus - immunology; Lactobacillus acidophilus; Lactobacillus casei; Lactobacillus delbrueckii; Lamina propria; Large intestine; Lymphocytes T; Macrophages; Mice; Mice, Inbred BALB C; Microbiota (Symbiotic organisms); Microorganisms; Mucosa; Peyer's Patches - immunology; Peyer's Patches - microbiology; Physiological aspects; Probiotics; Proteins - immunology; Small intestine; Streptococcus - immunology; Streptococcus thermophilus; T-Lymphocytes, Helper-Inducer - immunology; T-Lymphocytes, Helper-Inducer - microbiology; Tumor Necrosis Factor-alpha - immunology; Tumor necrosis factor-α; Argentina
• ISSN: 0954-3007; 1476-5640
• DOI: 10.1038/sj.ejcn.1601658

Health claims of lactic acid bacteria (LAB) used in functional foods and pharmaceutical preparations are based on the capacity of these microorganisms to stimulate the host immune system. In this study, the antigenic effect of LAB (Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus delbrueckii ssp. bulgaricus and Streptococcus thermophilus) on the gut immune system of BALB/c mice was evaluated. A dose-dependent increase of the Bcl2 protein was observed with all LAB assayed. Furthermore, the analysis of cytokine-producing cells in the lamina propria of gut showed that TNFalpha and INFgamma values, determined in macrophages cultured from Peyer patches, were enhanced for all the LAB assayed. An important increase of interleukins IL-10 and IL-4 was observed mainly in mice fed with Lactobacillus delbrueckii ssp. bulgaricus or Lactobacillus casei, while a significant induction of IL-2 and IL-12 was only observed with L. acidophilus (P<0.01). These effects were dose dependent. The role of produced cytokines in the balance Th1/Th2 was determined by a systemic antibody response against parenterally injected ovoalbumin. L. casei, L. delbrueckii ssp. bulgaricus and L. acidophilus enhanced the IgG1 response favouring Th2 balance, while L. acidophilus also increased the IgG2a response inducing Th1 balance. S. thermophilus did not influence the balance Th1/Th2. Our studies showed that lactic acid bacteria induce distinct mucosal cytokine profiles showing different adjuvant capacity among them. Thus, selection of probiotic strain with immunological properties must be well defined to influence cytokine expression that favour the claimed immune response.