Development of resistant transgenic soybeans with inverted repeat-coat protein genes of soybean dwarf virus

• Published in: Plant cell reports Vol. 25; no. 11; pp. 1213 - 1218
• Main Authors: Tougou, Makoto, Furutani, Noriyuki, Yamagishi, Noriko, Shizukawa, Yoshiaki, Takahata, Yoshihito, Hidaka, Soh
• Format: Journal Article
• Language: English
• Published: Berlin Berlin/Heidelberg : Springer-Verlag 01.11.2006; Springer; Springer Nature B.V
• Subjects: Aphididae; Biological and medical sciences; Biotechnology; Capsid Proteins - genetics; Fundamental and applied biological sciences. Psychology; Genetic engineering; Genetic technics; Glycine max - genetics; Glycine max - virology; Hybridization; Luteovirus - genetics; Methods. Procedures. Technologies; Plants, Genetically Modified - genetics; Plants, Genetically Modified - virology; Repetitive Sequences, Nucleic Acid; RNA Interference; RNA, Viral - metabolism; Seeds - genetics; Seeds - virology; Soybean dwarf virus; Soybeans; Transformation, Genetic; Transgenic animals and transgenic plants; Transgenic plants; Soybean dwarf virus; Transformation; Resistance; Small Interference RNA; Soybean; Glycine max; Virus; Resistance; Leguminosae; Gene; Dicotyledones; Angiospermae; Luteoviridae; Spermatophyta; Transgenic plant; Coat protein; Transgenesis; Glycine max; RNA silencing; SbDV
• ISSN: 0721-7714; 1432-203X
• DOI: 10.1007/s00299-006-0186-6

In an attempt to generate soybean plants resistant to soybean dwarf virus (SbDV), we transformed a construct containing inverted repeat-SbDV coat protein (CP) genes spaced by β-glucuronidase (GUS) sequences into soybean somatic embryos via microprojectile bombardment. Three T₀ plants with an introduced CP gene were obtained, and one generated T₁ seeds. The presence of the transgene in T₁ plants was confirmed by PCR and Southern blot hybridization analysis, but expression of CP was not detected by northern blot hybridization analysis. Two months after inoculation of SbDV by aphid, T₂ plants contained little SbDV-specific RNA and remained symptomless. These plants contained SbDV-CP-specific siRNA. These results suggest that the T₂ plants achieved resistance to SbDV by an RNA-silencing-mediated process.