New beamline dedicated to solution scattering from biological macromolecules at the ESRF

The new bio-SAXS beamline (ID14-3 at the ESRF, Grenoble, France) is dedicated exclusively to small-angle scattering experiments of biological macromolecules in solution and has been in user operation since November 2008. Originally a protein crystallography beamline, ID14-3 was refurbished, still as...

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Bibliographic Details
Published inJournal of physics. Conference series Vol. 247; no. 1; p. 012009
Main Authors Pernot, P, Theveneau, P, Giraud, T, Fernandes, R Nogueira, Nurizzo, D, Spruce, D, Surr, J, McSweeney, S, Round, A, Felisaz, F, Foedinger, L, Gobbo, A, Huet, J, Villard, C, Cipriani, F
Format Journal Article
LanguageEnglish
Published Bristol IOP Publishing 01.10.2010
Subjects
Automation
Crystallography
Data analysis
Data collection
Image reconstruction
Macromolecules
Physics
Small angle X ray scattering
Standard data
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Summary:The new bio-SAXS beamline (ID14-3 at the ESRF, Grenoble, France) is dedicated exclusively to small-angle scattering experiments of biological macromolecules in solution and has been in user operation since November 2008. Originally a protein crystallography beamline, ID14-3 was refurbished, still as a part of the ESRF Structural Biology group, with the main aim to provide a facility with 'quick and easy' access to satisfy rapidly growing demands from crystallographers, biochemists and structural biologists. The beamline allows manual and automatic sample loading/unloading, data collection, processing (conversion of a 2D image to a normalized 1D X-ray scattering profile) and analysis. The users obtain on-line standard data concerning the size (radius of gyration, maximum dimension and volume) and molecular weight of samples which allow on-the fly ab-inito shape reconstruction in order to provide feedback enabling the data collection strategies to be optimized. Automation of sample loading is incorporated on the beamline using a device constructed in collaboration between the EMBL (Grenoble and Hamburg outstations) and the ESRF. Semi/automated data analysis is implemented following the model of the SAXS facility at X33, EMBL Hamburg. This paper describes the bio-SAXS beamline and set-up characteristics together with the examples of user data obtained.
ISSN:1742-6596
1742-6588
1742-6596
DOI:10.1088/1742-6596/247/1/012009