Enzyme stabilization using synthetic compensatory solutes

The protective effect of the synthetic compensatory solutes, dimethylthetin (CAS 4727-41-7) and homodeanol betaine (N, N-dimethyl-N-(2-hydroxyethyl)-N-(2 carboxyethyl) ammonium inner salt, CAS 6249-53-2), on two enzymes: lactate dehydrogenase (LDH from rabbit muscle) and a microbial lipase, was comp...

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Published inBiocatalysis and biotransformation Vol. 23; no. 3-4; pp. 285 - 291
Main Authors Vasudevamurthy, Madhusudan K., Weatherley, Laurence R., Lever, Michael
Format Journal Article Conference Proceeding
LanguageEnglish
Published Abingdon Informa UK Ltd 01.01.2005
Taylor & Francis
Taylor and Francis
Subjects
Bioconversions. Hemisynthesis
Biological and medical sciences
Biotechnology
Freeze-drying
Fundamental and applied biological sciences. Psychology
heat-denaturation
lactate dehydrogenase (LDH)
lipase
Methods. Procedures. Technologies
synthetic compensatory solutes
lactate dehydrogenase (LDH)
Freeze drying
Enzyme
Stabilization
Denaturation
lipase
Triacylglycerol lipase
Esterases
L-Lactate dehydrogenase
Carboxylic ester hydrolases
Heat
synthetic compensatory solutes
Hydrolases
heat-denaturation
Oxidoreductases
Freeze-drying
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Summary:The protective effect of the synthetic compensatory solutes, dimethylthetin (CAS 4727-41-7) and homodeanol betaine (N, N-dimethyl-N-(2-hydroxyethyl)-N-(2 carboxyethyl) ammonium inner salt, CAS 6249-53-2), on two enzymes: lactate dehydrogenase (LDH from rabbit muscle) and a microbial lipase, was compared with that of glycine betaine, trehalose and sorbitol. When the enzyme plus 1 M solute were heated for 10 min at temperatures between 35-75°C, the temperature at which 50% of enzyme activity was lost increased most in the presence of trehalose (7.9° for LDH, 11.6° for lipase) and homodeanol betaine (10.7° for LDH, 11.0° for lipase). With both enzymes, more activity was retained at extreme temperatures in the presence of homodeanol betaine than with trehalose. Glycine betaine, dimethylthetin and sorbitol were less effective. Enzyme plus 1 M stabilizer solutions were frozen at −30°C and freeze-dried for 24 h. Trehalose was the most effective stabilizer of lactate dehydrogenase, and homodeanol betaine of lipase, during freeze-drying.
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ISSN:1024-2422
1029-2446
DOI:10.1080/10242420500190795