Development of a New In Vitro Methodology to Simulate Internal Root Resorption

• Published in: Journal of endodontics Vol. 40; no. 2; pp. 211 - 216
• Main Authors: da Silveira, Priscila Fernanda, DDS, Vizzotto, Mariana Boessio, DDS, MSc, PhD, Montagner, Francisco, DDS, MSc, PhD, Silveira, Heraldo Luis Dias da, DDS, MSc, PhD, Silveira, Heloísa Emília Dias da, DDS, MSc, PhD
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.02.2014
• Subjects: Cone-Beam Computed Tomography - methods; Cuspid - diagnostic imaging; Cuspid - drug effects; Cuspid - pathology; Dentin - diagnostic imaging; Dentin - drug effects; Dentin - pathology; Dentistry; Endocrinology & Metabolism; Humans; Image Processing, Computer-Assisted - methods; In Vitro Techniques; Incisor - diagnostic imaging; Incisor - drug effects; Incisor - pathology; Internal root resorption; methodology; Microscopy, Electron, Scanning; Nitric Acid - adverse effects; Odontometry - methods; Root Resorption - diagnostic imaging; Root Resorption - etiology; Root Resorption - pathology; scanning electron microscopy; Sodium Hypochlorite - adverse effects; Time Factors; Tomography, X-Ray Computed - methods; scanning electron microscopy; methodology; Internal root resorption
• ISSN: 0099-2399; 1878-3554
• DOI: 10.1016/j.joen.2013.07.007

Abstract Introduction Studies to evaluate the diagnostic power of imaging examinations to detect root resorption have been based on simulations produced by burs. Standardized, round, and well-outlined access cavities do not reproduce the characteristics of physiological lesions and may facilitate diagnosis, masking the true accuracy of imaging techniques. A methodology to simulate internal root resorption by using acid demineralization was developed. Methods Eleven extracted single-rooted teeth were mesiodistally sectioned into homologous halves. Root canals were labeled to ensure restricted and controlled action of fluids. The protocol was composed of 24-hour cycles (5% nitric acid for 12 hours, rinsing with deionized water, and 8% sodium hypochlorite for 10 minutes). At the end of each cycle 1 tooth was removed from treatment, defining an exposure time that cumulated to the last tooth. Electron microscopy imaging was assessed to determine lesion area and depth. Results Minimum and maximum and areas and depths were 3.14 mm² and 10.34 mm² and 0.22 mm and 1.59 mm, respectively. Resorption simulated by the protocol proposed reproduced lesions of different sizes. Conclusions The irregular shape and larger diameter:depth ratio suggest that these lesions are more similar to in vivo internal root resorption, compared with bur-induced lesions.