Regulation of Blastocyst Migration, Apposition, and Initial Adhesion by a Chemokine, Interferon γ-inducible Protein 10 kDa (IP-10), during Early Gestation

• Published in: The Journal of biological chemistry Vol. 278; no. 31; pp. 29048 - 29056
• Main Authors: Nagaoka, Kentaro, Nojima, Hisashi, Watanabe, Fumiko, Chang, Kyu-Tae, Christenson, Ronald K., Sakai, Senkiti, Imakawa, Kazuhiko
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.08.2003
• Subjects: Animals; Biotinylation; Blastocyst - physiology; Cell Adhesion; Cell Movement; Chemokine CXCL10; Chemokines, CXC - analysis; Chemokines, CXC - genetics; Chemokines, CXC - physiology; Endometrium - chemistry; Epithelial Cells - physiology; Female; Fibronectins - metabolism; Fluorescent Antibody Technique; Gene Expression - drug effects; Gestational Age; Goats; Integrins - genetics; Interferon Type I - pharmacology; Pregnancy; Pregnancy Proteins - pharmacology; Receptors, Chemokine - analysis; Receptors, Chemokine - metabolism; Receptors, CXCR3; Recombinant Proteins - metabolism; Recombinant Proteins - pharmacology; RNA, Messenger; Trophoblasts - cytology; Trophoblasts - physiology; Uterus - chemistry
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.M300470200

For a pregnancy to be established, initial apposition and adhesion of the blastocyst to maternal endometrium must occur in a coordinated manner; however, a key factor(s) that mediates the trophoblast cell migration and attachment to the apical surface of the endometrium has not been identified. In this study, we examined the effect of an endometrial chemokine, interferon-γ-inducible protein 10 kDa (IP-10), on conceptus migration to the endometrial epithelium. We first studied endometrial IP-10 mRNA expression, which was localized in the subepithelial stromal region, and detected the protein in the uterine flushing media during early pregnancy. Expression of IP-10 mRNA by the endometrium of cyclic animals was stimulated by the addition of a conceptus factor interferon-tau (IFN-τ). Immunofluorescent analysis revealed that IP-10 receptor, CXCR3, was localized in the trophoblast cells, to which biotinylated-recombinant caprine IP-10 (rcIP-10) bound. Chemotaxis assay indicated that rcIP-10 stimulated the migration of trophoblast cells, and the effects of rcIP-10 were neutralized by the pretreatment with an anti-IP-10 antibody. Adhesive activity of trophoblast cells to fibronectin was promoted by rcIP-10, and the effect was inhibited by the use of anti-IP-10 antibody. Further adhesion experiments demonstrated that binding of trophoblast cells to fibronectin was completely inhibited by a peptide of the Arg-Gly-Asp (RGD) sequence, which binds to integrins α5β1, αVβ1, αVβ3, and αVβ5, whereas non-binding peptide containing Arg-Gly-Glu (RGE) had minimal effects. More importantly, rcIP-10 promoted the adhesion of trophoblast cells to primary cells isolated from endometrial epithelium. Furthermore, rcIP-10 stimulated the expression of integrin α5, αV, and β3 subunit mRNA in trophoblast cells. These findings suggest that endometrial IP-10 regulates the establishment of apical interactions between trophoblast and epithelial cells during early gestation.