Human decidual natural killer cells are a unique NK cell subset with immunomodulatory potential
Natural killer cells constitute 50-90% of lymphocytes in human uterine decidua in early pregnancy. Here, CD56(bright) uterine decidual NK (dNK) cells were compared with the CD56(bright) and CD56(dim) peripheral NK cell subsets by microarray analysis, with verification of results by flow cytometry an...
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Published in | The Journal of experimental medicine Vol. 198; no. 8; pp. 1201 - 1212 |
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Main Authors | , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
The Rockefeller University Press
20.10.2003
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Subjects | |
Online Access | Get full text |
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Summary: | Natural killer cells constitute 50-90% of lymphocytes in human uterine decidua in early pregnancy. Here, CD56(bright) uterine decidual NK (dNK) cells were compared with the CD56(bright) and CD56(dim) peripheral NK cell subsets by microarray analysis, with verification of results by flow cytometry and RT-PCR. Among the approximately 10,000 genes studied, 278 genes showed at least a threefold change with P < or = 0.001 when comparing the dNK and peripheral NK cell subsets, most displaying increased expression in dNK cells. The largest number of these encoded surface proteins, including the unusual lectinlike receptors NKG2E and Ly-49L, several killer cell Ig-like receptors, the integrin subunits alpha(D), alpha(X), beta1, and beta5, and multiple tetraspanins (CD9, CD151, CD53, CD63, and TSPAN-5). Additionally, two secreted proteins, galectin-1 and progestagen-associated protein 14, known to have immunomodulatory functions, were selectively expressed in dNK cells. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 Abbreviations used in this paper: dNK, decidual NK; ITIM, immunoreceptor tyrosine-based inhibitory motif; KIR, killer cell Ig-like receptor; pNK, peripheral blood NK; PP14, progestagen-associated protein 14; PSG, pregnancy-specific glycoprotein. The online version of this article includes supplemental material. Address correspondence to Louise A. Koopman at her present address Biogen, Inc., 15 Cambridge Center, Rm. 8-530, Cambridge, MA 02142. Phone: (617) 679-3815; Fax: (617) 914-7140; email: louise_koopman@biogen.com; or Jack L. Strominger, Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138. Phone: (617) 495-3281; Fax: (617) 496-8351; email: jlstrom@fas.harvard.edu |
ISSN: | 0022-1007 1540-9538 |
DOI: | 10.1084/jem.20030305 |