AN EFFICIENT HPLC-UV METHOD FOR THE QUANTITATIVE DETERMINATION OF CEFADROXIL IN HUMAN PLASMA AND ITS APPLICATION IN PHARMACOKINETIC STUDIES

Cefadroxil is a semi-synthetic first-generation oral cephalosporin used in the treatment of mild to moderate infections of the respiratory and urinary tracts, skin and soft tissue infections. In this work a simple, rapid, economic and sensitive HPLC-UV method is described for the quantitative determ...

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Published inJournal of liquid chromatography & related technologies Vol. 35; no. 13; pp. 1871 - 1881
Main Authors Kano, Eunice Kazue, Serra, Cristina Helena dos Reis, Koono, Eunice Emiko Mori, Fukuda, Kazuo, Porta, Valentina
Format Journal Article
LanguageEnglish
Published Colchester Taylor & Francis Group 01.08.2012
Taylor & Francis
Taylor & Francis Ltd
Subjects
Analysis
Bioavailability
Biological and medical sciences
cefadroxil
General pharmacology
HPLC
human plasma
Liquid chromatography
Medical sciences
Pharmacokinetics
Pharmacology. Drug treatments
quantification
Human
Validation
Biological fluid
Chemical analysis
quantification
Healthy subject
Lactam
Oral administration
HPLC chromatography
Bioavailability
Blood
Blood plasma
Cephalosporin derivatives
Antibiotic
Reversed phase chromatography
Ultraviolet detector
Cefadroxil
Antibacterial agent
Pharmacokinetics
HPLC
human plasma
Quantitative analysis
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Summary:Cefadroxil is a semi-synthetic first-generation oral cephalosporin used in the treatment of mild to moderate infections of the respiratory and urinary tracts, skin and soft tissue infections. In this work a simple, rapid, economic and sensitive HPLC-UV method is described for the quantitative determination of cefadroxil in human plasma samples using lamivudine as internal standard. Sample pre-treatment was accomplished through protein precipitation with acetonitrile and chromatographic separation was performed with a mobile phase consisting of a mixture of sodium dihydrogen phosphate monohydrate solution, methanol and acetonitrile in the ratio of 90:8:2 (v/v/v) at a flow rate of 1.0 mL/min. The proposed method is linear between 0.4 to 40.0 µg/mL and its average recovery is 102.21% for cefadroxil and 97.94% for lamivudine. The method is simple, sensitive, reproducible, less time consuming for determination of cefadroxil in human plasma. The method can therefore be recommended for pharmacokinetics studies, including bioavailability and bioequivalence studies.
Bibliography:SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 14
ISSN:1082-6076
1520-572X
DOI:10.1080/10826076.2011.627607