The rpoN gene of Enterococcus faecalis directs sensitivity to subclass IIa bacteriocins

• Published in: Current microbiology Vol. 41; no. 6; pp. 441 - 443
• Main Authors: DALET, Karine, BRIAND, Cécilia, CENATIEMPO, Yves, HECHARD, Yann
• Format: Journal Article
• Language: English
• Published: New York, NY Springer 01.12.2000; Springer Nature B.V
• Subjects: Antibiotics; Bacteria; Bacteriocins - classification; Bacteriocins - pharmacology; Bacteriology; Biological and medical sciences; DNA-Binding Proteins; DNA-Directed RNA Polymerases - genetics; Drug Resistance, Microbial - genetics; Enterococcus faecalis; Enterococcus faecalis - drug effects; Enterococcus faecalis - genetics; Fundamental and applied biological sciences. Psychology; Genes, Bacterial; Genetic Complementation Test; Microbial Sensitivity Tests; Microbiology; Molecular Sequence Data; Mutagenesis, Insertional; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains; Restriction Mapping; RNA Polymerase Sigma 54; rpoN gene; s54 factor; Sequence Analysis, DNA; Sigma Factor - genetics; subclass IIa bacteriocins; Enterococcus faecalis; Resistance; Streptococcaceae; Nucleotide sequence; Bacteriocin; Initiation factor σ; Bacteria; Micrococcales
• ISSN: 0343-8651; 1432-0991
• DOI: 10.1007/s002840010164

The final sigma 54 factor has been previously described to be involved in Listeria monocytogenes sensitivity to mesentericin Y105, a subclass IIa bacteriocin. Here, we identified the rpoN gene, encoding final sigma 54, of Enterococcus faecalis JH2-2 and showed that its interruption leads to E. faecalis resistance to different subclass IIa bacteriocins. Moreover, this rpoN mutant remained sensitive to nisin, a class I bacteriocin, suggesting that final sigma 54 is especially involved in sensitivity to subclass IIa bacteriocins.