Tissue-engineered mucosa is a suitable model to quantify the acute biological effects of ionizing radiation

• Published in: International journal of oral and maxillofacial surgery Vol. 42; no. 8; pp. 939 - 948
• Main Authors: Tra, W.M.W, Tuk, B, van Neck, J.W, Hovius, S.E.R, Perez-Amodio, S
• Format: Journal Article
• Language: English
• Published: Denmark Elsevier Ltd 01.08.2013
• Subjects: Basement Membrane - radiation effects; Cell Adhesion - radiation effects; Cell Culture Techniques; Cell Differentiation - radiation effects; Cell Proliferation - radiation effects; Collagen Type IV - analysis; Collagen Type IV - radiation effects; Connective Tissue - radiation effects; cytokines; Dentistry; Desmoglein 3 - analysis; Desmoglein 3 - radiation effects; DNA damage; DNA Damage - radiation effects; DNA Repair - radiation effects; Epithelium - radiation effects; Female; Fibroblasts - radiation effects; Gamma Rays; Humans; Integrin alpha6 - analysis; Integrin alpha6 - radiation effects; Integrin beta4 - analysis; Integrin beta4 - radiation effects; Interleukin-1beta - analysis; Interleukin-1beta - radiation effects; Intracellular Signaling Peptides and Proteins - analysis; Intracellular Signaling Peptides and Proteins - radiation effects; Keratin-19 - analysis; Keratin-19 - radiation effects; keratinocytes; Keratinocytes - radiation effects; Male; Middle Aged; Mouth Mucosa - cytology; Mouth Mucosa - radiation effects; oral mucosa; Rad51 Recombinase - analysis; Rad51 Recombinase - radiation effects; Radiation Dosage; Surgery; Tissue Engineering; Transforming Growth Factor beta1 - analysis; Transforming Growth Factor beta1 - radiation effects; Tumor Suppressor p53-Binding Protein 1; Vimentin - analysis; Vimentin - radiation effects; tissue engineering; cytokines; keratinocytes; oral mucosa; DNA damage
• ISSN: 0901-5027; 1399-0020
• DOI: 10.1016/j.ijom.2013.01.025

Abstract The aim of this study was to evaluate the suitability of tissue-engineered mucosa (TEM) as a model for studying the acute effects of ionizing radiation (IR) on the oral mucosa. TEM and native non-keratinizing oral mucosa (NNOM) were exposed to a single dose of 16.5 Gy and harvested at 1, 6, 24, 48, and 72 h post-irradiation. DNA damage induced by IR was determined using p53 binding protein 1 (53BP1), and DNA repair was determined using Rad51. Various components of the epithelial layer, basement membrane, and underlying connective tissue were analyzed using immunohistochemistry. The expression of cytokines interleukin-1β (IL-1β) and transforming growth factor beta 1 (TGF-β1) was analyzed using an enzyme-linked immunosorbent assay. The expression of DNA damage protein 53BP1 and repair protein Rad51 were increased post-irradiation. The expression of keratin 19, vimentin, collage type IV, desmoglein 3, and integrins α6 and β4 was altered post-irradiation. Proliferation significantly decreased at 24, 48, and 72 h post-irradiation in both NNOM and TEM. IR increased the secretion of IL-1β, whereas TGF-β1 secretion was not altered. All observed IR-induced alterations in TEM were also observed in NNOM. Based on the similar response of TEM and NNOM to IR we consider our TEM construct a suitable model to quantify the acute biological effects of IR.