Mouse Adenovirus Type 1 Replication in Vitro Is Resistant to Interferon

• Published in: Virology (New York, N.Y.) Vol. 274; no. 1; pp. 213 - 219
• Main Authors: Kajon, Adriana E., Spindler, Katherine R.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.08.2000
• Subjects: Animals; Antiviral Agents - pharmacology; Cell Line; Drug Resistance, Microbial; E1A gene; Gene Expression - drug effects; Interferon-alpha - pharmacology; Interferon-beta - pharmacology; Interferon-gamma - pharmacology; Interferons - pharmacology; Mastadenovirus - drug effects; Mastadenovirus - physiology; Mice; Mouse adenovirus 1; Mutagenesis; Vesicular stomatitis Indiana virus - drug effects; Vesicular stomatitis Indiana virus - physiology; Virus Replication - drug effects
• ISSN: 0042-6822; 1096-0341
• DOI: 10.1006/viro.2000.0459

The effects of mouse interferon (IFN)-α/β and recombinant IFN-γ on mouse adenovirus type 1 (MAV-1) replication were investigated in single-cycle infectious virus yield reduction assays on mouse L929 cells. Viral yields at 3 days postinfection indicated that wt MAV-1 and pmE314, an early region 3 null mutant, were relatively insensitive to both IFN-α/β and IFN-γ, whereas early region 1A (E1A) mutants pmE109 (null), dlE105 (conserved region 1 deletion, CR1Δ), dlE102 (CR2Δ), and dlE106 (CR3Δ) were sensitive. MAV-1 E1A that was inducibly expressed in mouse fibroblast 37.1 cells rescued vesicular stomatitis virus from the antiviral effect of IFN-α/β but not from the antiviral effect of IFN-γ. Interferon-inducible gene expression was reduced in 37.1 cells as compared to the parental 3T6 cell line. Steady-state levels of IFN-inducible gene mRNAs were also reduced in 3T6 cells infected with the wild-type virus and pmE314 but not in cells infected with pmE109. These results suggest that the MAV-1 E1A gene product is capable of interfering with the signaling pathways of both types of IFN, although modulation of IFN-α/β antiviral activity was more pronounced.