Gz- and not Gi-proteins are coupled to pre-junctional μ-opioid receptors in bovine airways

• Published in: Respiratory physiology & neurobiology Vol. 189; no. 1; pp. 162 - 166
• Main Authors: Baroffio, Michele, Brichetto, Lorenzo, Franco, Luisa, Crimi, Emanuele, Rehder, Kai, Brusasco, Vito
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.10.2013
• Subjects: Acetylcholine; Acetylcholine - secretion; Analgesics, Opioid - pharmacology; Animals; Arachidonic acid; Arachidonic Acid - pharmacology; Blotting, Western; Bovine trachealis; Cattle; Electric Stimulation; Enkephalin, Ala-MePhe-Gly- - pharmacology; G proteins; GTP-Binding Protein alpha Subunits, Gi-Go - metabolism; Medical Education; Muscle, Smooth - drug effects; Muscle, Smooth - metabolism; Neurotransmission; Pertussis toxin; Pulmonary/Respiratory; Receptors, Opioid, mu - metabolism; Signal Transduction - drug effects; Signal Transduction - physiology; Trachea - drug effects; Trachea - metabolism; Acetylcholine; G proteins; Bovine trachealis; Arachidonic acid; Neurotransmission; Pertussis toxin
• ISSN: 1569-9048; 1878-1519
• DOI: 10.1016/j.resp.2013.07.021

Highlights • The effect of μ-opioid agonist DAMGO on acethylcholine (ACh) release was studied. • Arachidonic acid but not pertussis toxin reduced the inhibitory effect of DAMGO. • Arachidonic acid alone inhibited ACh release by opening Ca2+ -dependent K+ -channels. • Gz α-subunit was consistently expressed in tracheal tissue samples. • In bovine trachealis, μ-opioids inhibit ACh-release via Gz- rather than Gi-protein.