Rat tropoelastin is synthesized from a 3.5-kilobase mRNA

A lambda gt11 cDNA library was constructed from poly(A+) RNA isolated from aortic tissue of neonatal rats and screened with a human tropoelastin cDNA clone. DNA sequence analysis of several overlapping rat clones confirmed the presence of DNA sequences coding for murine tropoelastin and DNA sequence...

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Published inThe Journal of biological chemistry Vol. 263; no. 27; pp. 13504 - 13507
Main Authors Deak, S B, Pierce, R A, Belsky, S A, Riley, D J, Boyd, C D
Format Journal Article
LanguageEnglish
Published Bethesda, MD Elsevier Inc 25.09.1988
American Society for Biochemistry and Molecular Biology
Subjects
Animals
Animals, Newborn
Aorta - analysis
Bacteriophage lambda - genetics
Base Sequence
Biological and medical sciences
DNA - genetics
DNA, Recombinant
Elastin - analogs & derivatives
Fundamental and applied biological sciences. Psychology
Gene expression
Humans
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Nucleic Acid Hybridization
Protein Biosynthesis
Rats
RNA, Messenger - genetics
Sequence Homology, Nucleic Acid
Transcription, Genetic
Tropoelastin - biosynthesis
Tropoelastin - genetics
Molecular hybridization
Vertebrata
Mammalia
Messenger RNA
Rat
Nucleotide sequence
Elastin
Rodentia
Extracellular matrix
Complementary RNA
Comparative study
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Summary:A lambda gt11 cDNA library was constructed from poly(A+) RNA isolated from aortic tissue of neonatal rats and screened with a human tropoelastin cDNA clone. DNA sequence analysis of several overlapping rat clones confirmed the presence of DNA sequences coding for murine tropoelastin and DNA sequences coding for the 3′-untranslated region of the rat tropoelastin mRNA. Northern blot analysis of total RNA from aortic tissue of neonatal rats using oligonucleotide probes derived from these rat tropoelastin cDNAs demonstrated the presence of a 3.5-kilobase tropoelastin mRNA. The size of this rat tropoelastin mRNA agrees with previous reports for the size of the mRNA coding for tropoelastin in tissue from several vertebrate species but contrasts with several reports suggesting the presence of a higher molecular weight mRNA species responsible for the synthesis of tropoelastin in rodent tissue.
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ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(18)68269-9