Characterization and Effectiveness of Co-inoculation of Sinorhizobium Strains on Annual Medics
Selection and identification of better-adapted annual medics with an appropriate rhizobia inoculum is crucial for improved forage quality and yield. The objectives of this study were to characterize 11 rhizobial strains used for an effectiveness study on annual medics and generate an inoculum blend...
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Published in | Crop science Vol. 52; no. 2; pp. 932 - 942 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Madison, WI
Crop Science Society of America
01.03.2012
The Crop Science Society of America, Inc |
Subjects | |
Online Access | Get full text |
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Summary: | Selection and identification of better-adapted annual medics with an appropriate rhizobia inoculum is crucial for improved forage quality and yield. The objectives of this study were to characterize 11 rhizobial strains used for an effectiveness study on annual medics and generate an inoculum blend suitable for annual medic species adapted to the southern Great Plains. Each strain was compared to reference rhizobia strains by polymerase chain reaction (PCR) based on repetitive sequences (rep-PCR) and ribosomal gene sequences and analyzed for plant growth promoting (PGP) activities. Genetic characterization separated the strains into two groups belonging to Sinorhizobium medicae (seven isolates) and Sinorhizobium meliloti (four isolates). Three of the 11 strains tested positive for the PGP activities of indole acetic acid production and phosphate solubilization. A blend of three strains (NFmix3, consisting of WSM1115, 102F85, and M49), of which two were positive for PGP activities, was used in an effectiveness study for the annual medics Medicago minima (L.) Bartal., Medicago orbicularis (L.) Bartal., Medicago rigidula (L.) All., and Medicago rigiduloides E. Small. The NFmix3 association with three of the four annual medic species resulted in equivalent biomass production as that of the single specific strain. The NFmix3 blend would be suitable and more economic for use with a mixture of medic species that may provide expansion of the grazing season. |
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Bibliography: | http://dx.doi.org/10.2135/cropsci2011.08.0459 All rights reserved. No part of this periodical may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopying, recording, or any information storage and retrieval system, without permission in writing from the publisher. Permission for printing and for reprinting the material contained herein has been obtained by the publisher. |
ISSN: | 1435-0653 0011-183X 1435-0653 |
DOI: | 10.2135/cropsci2011.08.0459 |