Molecular identification of four novel cytochrome P450 genes related to the development of resistance of Spodoptera exigua (Lepidoptera: Noctuidae) to chlorantraniliprole

• Published in: Pest management science Vol. 74; no. 8; pp. 1938 - 1952
• Main Authors: Wang, Xuegui, Chen, Yiqu, Gong, Changwei, Yao, Xinge, Jiang, Chunxian, Yang, Qunfang
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.08.2018; Wiley Subscription Services, Inc
• Subjects: Amino Acid Sequence; Animals; chlorantraniliprole; Cytochrome; Cytochrome P-450 Enzyme System - chemistry; Cytochrome P-450 Enzyme System - genetics; Cytochrome P-450 Enzyme System - metabolism; Cytochrome P450; cytochrome P450 monooxygenase; Double-stranded RNA; Feeding; Gene expression; Gene Silencing; Genes; Insect Proteins - chemistry; Insect Proteins - genetics; Insect Proteins - metabolism; Insecticide resistance; Insecticide Resistance - genetics; Insecticides; Insecticides - pharmacology; Insects; Larva - drug effects; Larva - genetics; Larva - growth & development; Molecular chains; Organic chemistry; ortho-Aminobenzoates - pharmacology; Phylogeny; Polymerase chain reaction; Populations; Proteins; Receptors; Reverse transcription; Ribonucleic acid; RNA; RNA interference; RNA, Double-Stranded - genetics; RNA-mediated interference; Ryanodine receptors; Sequence Alignment; Spodoptera - drug effects; Spodoptera - enzymology; Spodoptera - genetics; Spodoptera - growth & development; Spodoptera exigua; transcriptome; RNA interference; cytochrome P450 monooxygenase; chlorantraniliprole; transcriptome; Spodoptera exigua
• ISSN: 1526-498X; 1526-4998
• DOI: 10.1002/ps.4898

BACKGROUND The beet armyworm, Spodoptera exigua, is an omnivorous insect that damages a variety of crops worldwide. Chlorantraniliprole is a new diamide insecticide that acts on the ryanodine receptors in insects. The aim of this study was to explore key genes related to the development of chlorantraniliprole resistance in S. exigua. RESULTS Transcriptomes were compared between beet armyworms from a susceptible laboratory strain (Sus‐Lab) and Sus‐Lab screened with LC25 sublethal doses of chlorantraniliprole for six generations (SE‐Sel). Ten of 11 cytochrome P450 genes with upregulated expression verified by quantitative reverse transcription polymerase chain reaction (qRT‐PCR) in SE‐Sel strains were detected in SE‐PZ16 and SE‐ZY16, two extremely resistant field populations. In addition, expression of four new cytochrome P450 genes, CYP9A21v1, CYP9A21v2, CYP9A21v3 and CYP9A21v4, was shown in the two field populations and was significantly higher in the SE‐Sel strain than in the Sus‐Lab strain (P < 0.05). Their full‐length and protein tertiary structures were also cloned and predicted. The function of CYP9A21v3 was analysed by RNA interference, and the relative expression of CYP9A21v3 in the SE‐ZY16 population after feeding on dsRNA was lower than in the control group. Moreover, mortality rates in insects treated at the LC50 of chlorantraniliprole after dsRNA feeding were significantly higher than in the control group 24 h after treatment (P < 0.05). CONCLUSION Overexpression of CYP9A21v3 may be a primary factor in the development of chlorantraniliprole resistance in beet armyworms. © 2018 Society of Chemical Industry The aim of this study was to explore the key genes related to the development of resistance to chlorantraniliprole in Spodoptera exigua.