2-DE analysis of a new human cell line EM-G3 derived from breast cancer progenitor cells and comparison with normal mammary epithelial cells

• Published in: Proteomics (Weinheim) Vol. 7; no. 9; pp. 1549 - 1559
• Main Authors: Selicharová, Irena, Smutná, Kateřina, Šanda, Miloslav, Ubik, Karel, Matoušková, Eva, Buršíková, Eva, Brožová, Markéta, Vydra, Jan, Jiráček, Jiří
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 01.05.2007; WILEY‐VCH Verlag; Wiley-VCH
• Subjects: 2-DE; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Breast cancer; Breast Neoplasms - chemistry; Carcinoma, Ductal, Breast - chemistry; Cell Line, Tumor; Electrophoresis, Gel, Two-Dimensional; EM-G3 cell line; Fundamental and applied biological sciences. Psychology; Gynecology. Andrology. Obstetrics; Humans; Mammary gland diseases; Mammary Glands, Human - chemistry; Mammary Glands, Human - cytology; Medical sciences; Miscellaneous; Neoplasm Proteins - chemistry; Phenotype; Progenitors; Proteins; Proteome - chemistry; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Stem Cells - chemistry; Tumors; Human; EM-G3 cell line; Mammary gland diseases; Cell line; Proteomics; Epithelial cell; Breast cancer; Malignant tumor; 2-DE; Progenitors; Progenitor cell
• ISSN: 1615-9853; 1615-9861
• DOI: 10.1002/pmic.200600907

We performed a 2‐DE analysis of proteins of the newly established spontaneously immortalized clonal cell line EM‐G3 derived from a primary lesion of infiltrating ductal breast carcinoma. EM‐G3 cells may represent progenitors of the mammary epithelial cells spontaneously immortalized in early phase of cancerogenesis. We compared the protein profile of EM‐G3 line with proteins from populations of normal mammary epithelial cells (NME), and determined the phenotype of both types of cells. NME cells are a mixture of both main cell types in breast epithelia, myoepithelial and luminal cells. The EM‐G3 breast cancer cell line has a unique basal‐like phenotype. We identified proteins that are differently expressed in these cells. Cytokeratin 16, cytokeratin 19, squamous cell carcinoma antigen 1, caphepsin B and caspase 14 were predominantly expressed by NME cells. Cytokeratin 13, isoelectric variant of annexin 5, isoelectric variant of chloride intracellular channel protein 1, glyoxalase 1 and glutamine synthetase were predominantly expressed by EM‐G3 cells. The proteins up‐regulated in EM‐G3 cells may represent potential protein markers of mammary epithelial cells progenitors and may be important in early phase of carcinogenesis.