Proteomics analysis revealed changes in rat bronchoalveolar lavage fluid proteins associated with oil mist exposure

• Published in: Proteomics (Weinheim) Vol. 6; no. 7; pp. 2236 - 2250
• Main Authors: Lee, Yung-Shan, Chen, Pang-Wei, Tsai, Perng-Jy, Su, Shu-Hui, Liao, Pao-Chi
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 01.04.2006; WILEY‐VCH Verlag; Wiley-VCH
• Subjects: Aerosols; Amino Acid Sequence; Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Bronchoalveolar lavage fluid; Bronchoalveolar Lavage Fluid - chemistry; Chromatography, Liquid; Electrophoresis, Gel, Two-Dimensional; Environmental Exposure - adverse effects; Fundamental and applied biological sciences. Psychology; Industrial Oils - adverse effects; Liquid chromatography-tandem mass spectrometry; Male; Mass Spectrometry; Miscellaneous; Molecular Sequence Data; Oil mist exposure; Proteins; Proteins - analysis; Proteins - chemistry; Proteomics; Rats; Rats, Sprague-Dawley; Vertebrata; Mammalia; Rat; Bronchoalveolar lavage; uid chromatography-tandem mass spectrometry / Oil mist exposure; Rodentia; Proteomics; Bronchoalveolar lavage fluid; Liquid chromatography; Mass spectrometry; Protein
• ISSN: 1615-9853; 1615-9861
• DOI: 10.1002/pmic.200500347

Exposure to oil mist has been associated with a variety of acute and chronic respiratory effects. Using proteomics approaches to investigate exposure‐associated proteins may provide useful information to understand the mechanisms of associated respiratory effects. The aim of this study was to investigate changes in rat bronchoalveolar lavage fluid proteins associated with oil mist exposure using nano‐HPLC‐ESI‐MS/MS. The results revealed that 29 proteins exhibited significant changes after exposure. These proteins included surfactant‐associated proteins (SP‐A and SP‐D), inflammatory proteins (complement component 3, immunoglobulins, lysozyme, etc.), growth factors (e.g., transforming growth factor alpha (TGF‐α)), calcium‐binding proteins (calcyclin, calgranulin A, calreticulin, and calvasculin), and other proteins (e.g., cathepsin D, saposin, and intestinal trefoil factor). To further evaluate changes in protein levels, a simple quantitative strategy was developed in this study. A large decrease in protein levels of SP‐A and SP‐D (0.24‐ and 0.38‐fold, respectively) following exposure was observed. In contrast, protein levels of TGF‐α and calcium‐binding proteins were significantly increased (4.46‐ and 1.4–1.8‐fold, respectively). Due to the diverse functions of these proteins, the results might contribute to understand the mechanisms involved in lung disorders induced by oil mist exposure.