Survival of Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium in manure and manure‐amended soil under tropical climatic conditions in Sub‐Saharan Africa

• Published in: Journal of applied microbiology Vol. 110; no. 4; pp. 1007 - 1022
• Main Authors: Ongeng, D., Muyanja, C., Geeraerd, A.H., Springael, D., Ryckeboer, J.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.04.2011; Blackwell; Oxford University Press
• Subjects: Africa South of the Sahara; Agriculture; Biological and medical sciences; E coli; E. coli O157:H7; Escherichia coli; Escherichia coli O157 - growth & development; Fundamental and applied biological sciences. Psychology; manure; Manure - microbiology; Manures; manure‐amended soil; Microbial Viability; Microbiology; Salmonella; Salmonella enterica; Salmonella Typhimurium; Salmonella typhimurium - growth & development; Soil Microbiology; survival; Tropical Climate; tropics; Africa South of the Sahara; Climatic condition; Soils; manure-amended soil; manure; Applied microbiology; Escherichia coli; tropics; Bacteria; E. coli O157:H7; Salmonella typhimurium; Survival; Enterobacteriaceae; E. coli O157:H7; Salmonella Typhimurium; survival
• ISSN: 1364-5072; 1365-2672
• DOI: 10.1111/j.1365-2672.2011.04956.x

Aims:  To establish the fate of Escherichia coli O157:H7 and Salmonella Typhimurium in manure and manure‐amended agricultural soils under tropical conditions in Sub‐Saharan Africa. Methods and Results:  Survival of nonvirulent E. coli O157:H7 and Salm. Typhimurium at 4 and 7 log CFU g−1 in manure and manure‐amended soil maintained at ≥80% r.h. or exposed to exclusive field or screen house conditions was determined in the Central Agro‐Ecological Zone of Uganda. Maintaining the matrices at high moisture level promoted the persistence of high‐density inocula and enhanced the decline of low‐density inocula in the screen house, but moisture condition did not affect survival in the field. The large majority of the survival kinetics displayed complex patterns corresponding to the Double Weibull model. The two enteric bacteria survived longer in manure‐amended soil than in manure. The 7 log CFU g−1E. coli O157:H7 and Salm. Typhimurium survived for 49–84 and 63–98 days, while at 4 log CFU g−1, persistence was 21–28 and 35–42 days, respectively. Conclusions:  Under tropical conditions, E. coli O157:H7 and Salm. Typhimurium persisted for 4 and 6 weeks at low inoculum density and for 12 and 14 weeks at high inoculum density, respectively. Significance and Impact of the Study:  Persistence in the tropics was (i) mostly shorter than previously observed in temperate regions thus suggesting that biophysical conditions in the tropics might be more detrimental to enteric bacteria than in temperate environments; (ii) inconsistent with published data isothermally determined previously hence indicating the irrelevance of single point isothermal data to estimate survival under dynamic temperature conditions.