Paenibacillus marinum sp. nov., a thermophilic xylanolytic bacterium isolated from a marine hot spring in Tunisia

• Published in: Journal of basic microbiology Vol. 53; no. 11; pp. 877 - 883
• Main Authors: Bouraoui, Hanene, Rebib, Hanen, Aissa, Melek Ben, Touzel, Jean Pierre, O'donohue, Michael, Manai, Mohamed
• Format: Journal Article
• Language: English
• Published: Germany Blackwell Publishing Ltd 01.11.2013; Wiley-VCH Verlag
• Subjects: Bacteria; Bacterial Typing Techniques; Base Composition; Cell Wall - chemistry; Cluster Analysis; Cytoplasm - chemistry; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Fatty Acids - analysis; Hot Springs - microbiology; Hydrolysis; Life Sciences; Marine hot spring; Mediterranean Sea; Microscopy; Molecular Sequence Data; Paenibacillus; Paenibacillus - classification; Paenibacillus - genetics; Paenibacillus - isolation & purification; Paenibacillus - metabolism; Paenibacillus marinum; Phylogeny; Quinones - analysis; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Spores, Bacterial - cytology; Thermophile; Tunisia; Xylan; Xylans - metabolism; Mediterranean Sea; Tunisia; Xylan; Paenibacillus marinum; Marine hot spring; Thermophile; renaturation rate; xylan; systematic; thermophile; xylanase; diversity; marine hot spring; performance liquid chromatography; dna hybridization; paenibacillus marinum; phoenix dactylifera; phyllosphere; cell wall; alignment
• ISSN: 0233-111X; 1521-4028
• DOI: 10.1002/jobm.201200275

Among a large collection of Tunisian hot springs bacterial isolates a bacterial strain, THE22T, with xylanolytic properties was identified. The bacterium was isolated from a natural hot spring “Ain Echefa” at Mediteranean sea (Korbous, North‐Eastern Tunisia). The novel strain was Gram positive, spore‐forming, rod‐shaped, facultatively anaerobic and grew optimally under conditions of 55 °C, 1% (w/v) NaCl and pH 7–8. The 16S rRNA gene sequence analysis showed that strain THE22T fell within the radiation of the cluster comprising Paenibacillus species with Paenibacillus phyllosphaerae PALXIL04T as the closest phylogenetic neighbour (95.8%). The predominant components in the fatty methyl ester profile were iso‐C16:0 (34.46%), C16:0 (19.64%), anteiso‐C15:0 (19.18%) and anteiso‐C17:0 (18.11%). The major respiratory quinone was menaquinone‐7 (MK‐7). The diamino acid found in the cell‐wall peptidoglycan was meso‐diaminopimelic acid. The base composition of DNA was 56 mol%. Based on the polyphasic taxonomic data, strain THE‐22T (=DSM 18499T = LMG 23758T) was recognized as a novel species within the genus Paenibacillus. The name Paenibacillus marinum sp. nov. is proposed.