Low Trypsin and Chymotrypsin Inhibitor Mutants in Winged Bean(P sophocarpus tetragonolobus(L) DC)

• Published in: Journal of the science of food and agriculture Vol. 71; no. 1; pp. 137 - 140
• Main Authors: Kothekar, Vijay S, Harsulkar, Abhay M, Khandelwal, Ajay R
• Format: Journal Article
• Language: English
• Published: London John Wiley & Sons, Ltd 01.05.1996; Wiley; Published for the Society of Chemical Industry by Elsevier Applied Science
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; chymotrypsin inhibitor; Fundamental and applied biological sciences. Psychology; Genetics and breeding of economic plants; mutants; trypsin inhibitor; Varietal selection. Specialized plant breeding, plant breeding aims; winged bean; Yield, quality, earliness, varia; Psophocarpus tetragonolobus; Laboratory study; Serine endopeptidases; Enzyme; Nutritive value; Genotype; Metabolic inhibitor; Proteins; Grain legume; Chymotrypsin; Trypsin; Leguminosae; Enzymatic activity; Dicotyledones; Content; Angiospermae; Genetic improvement; Hydrolases; Spermatophyta; Mutation; Proteinases; Digestibility
• ISSN: 0022-5142; 1097-0010
• DOI: 10.1002/(SICI)1097-0010(199605)71:1<137::AID-JSFA563>3.0.CO;2-Y

Low trypsin inhibitor (LTI) and chymotrypsin inhibitor (LCTI) mutants have been identified and isolated from the M5 mutant lines of winged bean. The LTI and LCTI lines demonstrated a true breeding feature in the subsequent generations. Three to five isoinhibitor bands of trypsin and chymotrypsin were absent in different mutants, indicating mutations in the respective gene loci. The LTI and LCTI mutants inhibited insect gut proteases as efficiently as their control counterparts. This could be attributed to a specific group of inhibitors present in all the mutant types.