Multiparametric analysis of amino acids and organic acids in rat brain tissues using GC/MS

• Published in: Journal of separation science Vol. 31; no. 15; pp. 2831 - 2838
• Main Authors: Lin, Jingchao, Su, Mingming, Wang, Xiaoyan, Qiu, Yunping, Li, Houkai, Hao, Jin, Yang, Hongzhou, Zhou, Mingmei, Yan, Chao, Jia, Wei
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 01.08.2008; WILEY‐VCH Verlag; Wiley
• Subjects: Amino acid; Amino Acids - analysis; Aminoacids, peptides. Hormones. Neuropeptides; Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Brain tissue; Calibration; Carboxylic Acids - analysis; Cerebral Cortex - chemistry; Extraction; Fundamental and applied biological sciences. Psychology; Gas Chromatography-Mass Spectrometry - instrumentation; Gas Chromatography-Mass Spectrometry - methods; GC/MS; Hippocampus - chemistry; Intermediary metabolites. Miscellaneous; Multivariate Analysis; Organic acid; Other biological molecules; Proteins; Rats; Rats, Wistar; Reproducibility of Results; Sensitivity and Specificity; Thalamus - chemistry; Chemical analysis; Rat; Organic acid; Central nervous system; Multivariate analysis; Derivatization; Solvent extraction; Organic acids; Encephalon; Tissue; Sample preparation; Amino acid; Extraction; Quantitative analysis; Validation; Statistical analysis; Coupled method; Rodentia; Brain tissue; Esterification; Gas chromatography; α-Aminoacid; Vertebrata; Mammalia; Multicomponent analysis; Animal; Simultaneous measurement; Mass spectrometry; Chemometrics; GC/MS; Principal component analysis
• ISSN: 1615-9306; 1615-9314
• DOI: 10.1002/jssc.200800232

Using design of experiment (DOE) theory coupled with multivariate statistical analysis, we have developed a simple and reliable GC/MS‐based analytical assay for simultaneous analysis of amino acids and organic acids in rat brain tissue samples. The process of water extraction (pH 10.0) was extensively evaluated using brain tissue samples and a set of 21 reference standards. Acceptable calibration curves were obtained over a wide concentration range, 0.2–35.0 μg/mL for standards and 15.0–2.4 mL/g (tissue) for brain tissue samples. The precision was mostly better than 10% for both the mixed standards and the brain tissue samples. The brain tissue samples exhibited good stability within 48 h with RSD generally less than 15%. Furthermore, the developed analytical method was successfully applied in distinguishing the subtle variation among different parts of the brain tissues, such as cerebral cortex, hippocampus, and thalamus.