Direct simultaneous determination of eight sweeteners in foods by capillary isotachophoresis
A method for isotachophoretic determination of sweeteners of different character in candies and chewing gums was developed. A capillary of 0.8 mm ID and 90 mm effective length made of fluorinated ethylene‐propylene copolymer is filled with an electrolyte system consisting of 10 mM HCl + 14 mM Tris,...
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Published in | Journal of separation science Vol. 29; no. 8; pp. 1132 - 1137 |
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Main Authors | , , , |
Format | Journal Article Conference Proceeding |
Language | English |
Published |
Weinheim
WILEY-VCH Verlag
01.05.2006
WILEY‐VCH Verlag Wiley |
Subjects | |
Online Access | Get full text |
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Summary: | A method for isotachophoretic determination of sweeteners of different character in candies and chewing gums was developed. A capillary of 0.8 mm ID and 90 mm effective length made of fluorinated ethylene‐propylene copolymer is filled with an electrolyte system consisting of 10 mM HCl + 14 mM Tris, pH 7.7 (leading electrolyte) and 5 mM L‐histidine + 5 mM Tris, pH 8.3 (terminating electrolyte). The analysis is performed at a driving current of 200 μA and for detection current is decreased to 100 μA. Boric acid is added to the aqueous sample solution to form borate complexes with substances of polyhydroxyl nature and make them migrate isotachophoretically. Using conductivity detection, the calibration curves in the tested concentration range up to 2.5 mM were linear for all components of interest: acesulfame K, saccharine, aspartame, cyclamate, sorbitol, mannitol, lactitol, and xylitol. The concentration detection limits ranged between 0.024 and 0.081 mM. Good precision of the ITP method is evidenced by favorable RSD values ranging from 0.8 to 2.8% obtained at the analyte concentration of 1.0 mM (n = 6). The analysis time was about 20 min. Simplicity, accuracy, and low cost of analyses make ITP an alternative procedure to methods used so far for the determination of ionizable sweeteners. |
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Bibliography: | ark:/67375/WNG-BH0VGMXB-6 ArticleID:JSSC200500441 istex:7599CE1511E139F692D115A77439A95C5919C195 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1615-9306 1615-9314 |
DOI: | 10.1002/jssc.200500441 |