Mandibular bone remodeling under a choline-deficient diet: a histomorphometric study in rats

• Published in: Journal of periodontology (1970) Vol. 74; no. 6; p. 831
• Main Authors: Gorustovich, Alejandro A, Espósito, María A, Guglielmotti, María B, Giglio, Máximo J
• Format: Journal Article
• Language: English
• Published: United States 01.06.2003
• Subjects: Analysis of Variance; Animals; Body Weight - drug effects; Bone Density - drug effects; Bone Remodeling - drug effects; Choline - pharmacology; Coloring Agents; Diet; Image Processing, Computer-Assisted; Lipotropic Agents - pharmacology; Male; Mandible - drug effects; Mandible - pathology; Osteoblasts - drug effects; Osteoblasts - pathology; Osteogenesis - drug effects; Rats; Rats, Wistar
• ISSN: 0022-3492
• DOI: 10.1902/jop.2003.74.6.831

A deficiency of lipotropic factors in the rat induces renal, hepatic, and/or hematic damage. The aim of the present study was to evaluate the effect of a choline-deficient diet and refeeding on mandibular bone remodeling. Fifty Wistar rats were divided into 5 groups: group 1 (G1): control diet for 15 days; group 2 (G2): choline-deficient diet for 15 days; group 3 (G3): control diet for 30 days; group 4 (G4): choline-deficient diet for 30 days; and group 5 (G5): choline-deficient diet for 15 days and control diet for 15 days. All animals were sacrificed by ether overdose. The mandibles were resected, radiographed, decalcified, processed, and embedded in paraffin. Bucco-lingually oriented sections were obtained at the level of the interradicular bone of the medial roots of the left first molar, and stained with hematoxylin and eosin (H & E). Bone tissue density and bone remodeling were determined histomorphometrically. Body weight, food intake, hematocrit, and hemoglobinemia were also recorded. Microscopic observation revealed that osteogenesis was lower in rats fed a choline-deficient diet, at both 15 and 30 days, and that this decrease did not revert with a control diet. Histomorphometric evaluation showed 37% and 27% reduction in bone tissue density at 15 and 30 days, respectively, and a 30% decrease in bone formation at 30 days, compared to controls. In this experimental model, a choline-deficient diet led to altered bone remodeling as observed by a marked reduction in osteogenesis.