Application of NMR Spectroscopy in the Assessment of Radiation Dose in Human Primary Cells

• Published in: Chemistry & biodiversity Vol. 12; no. 11; pp. 1696 - 1705
• Main Authors: Kang, Chang-Mo, Seong Hyeon, Jin, Ra Kim, So, Kyeong Lee, Eun, Jin Yun, Hyun, Young Kim, Sun, Kee Chae, Young
• Format: Journal Article
• Language: English
• Published: Zürich WILEY-VCH Verlag 01.11.2015; WILEY‐VCH Verlag; Wiley Subscription Services, Inc
• Subjects: Alanine - metabolism; Dose-Response Relationship, Radiation; Food irradiation; Glutamic Acid - metabolism; Glycine - metabolism; Humans; Magnetic Resonance Spectroscopy; Metabolite profiling; Metabolites; NMR Spectroscopy; Primary cell; Primary Cell Culture; Spectrum analysis; X-Ray irradiation; X-Rays; NMR Spectroscopy; Primary cell; Metabolite profiling; X-Ray irradiation
• ISSN: 1612-1872; 1612-1880
• DOI: 10.1002/cbdv.201400431

We employed the primary cell model system as a first step toward establishing a method to assess the influence of ionizing radiation by using a combination of common and abundant metabolites. We applied X‐ray irradiation amounts of 0, 1, and 5 Gy to the cells that were harvested 24, 48, or 72 h later, and profiled metabolites by 2D‐NMR spectroscopy to sort out candidate molecules that could be used to distinguish the samples under different irradiation conditions. We traced metabolites stemming from the input 13C‐glucose, identified twelve of them from the cell extracts, and applied statistical analysis to find out that all the metabolites, including glycine, alanine, and gluatamic acid, increased upon irradiation. The combinatorial use of the selected metabolites showed promising results where the product of signal intensities of alanine and lactate could differentiate samples according to the dose of X‐ray irradiation. We hope that this work can form a base for treating radiation‐poisoned patients in the future.