GLUTATHIONE S‐TRANSFERASE Genes IN THE RICE LEAFFOLDER, Cnaphalocrocis medinalis (LEPIDOPTERA: PYRALIDAE): IDENTIFICATION AND EXPRESSION PROFILES

• Published in: Archives of insect biochemistry and physiology Vol. 90; no. 1; pp. 1 - 13
• Main Authors: Liu, Su, Rao, Xiang‐Jun, Li, Mao‐Ye, Feng, Ming‐Feng, He, Meng‐Zhu, Li, Shi‐Guang
• Format: Journal Article
• Language: English
• Published: United States Alan R. Liss 01.09.2015; Blackwell Publishing Ltd; Wiley Subscription Services, Inc
• Subjects: abdomen; adults; Animals; chlorpyrifos; Chlorpyrifos - pharmacology; Cnaphalocrocis medinalis; data collection; differential gene expression; fat body; Gene Expression Profiling; gene expression regulation; Genes; Genes, Insect; glutathione S-transferases (GST); glutathione transferase; Glutathione Transferase - genetics; Inactivation, Metabolic - genetics; insecticide detoxification; insecticide resistance; Insecticide Resistance - genetics; Insecticides - pharmacology; insects; Larva - drug effects; Larva - enzymology; Larva - genetics; larvae; Lepidoptera; Malpighian tubules; midgut; Moths - drug effects; Moths - enzymology; Moths - genetics; pests; Phylogeny; proteins; Pyralidae; quantitative polymerase chain reaction; rice; Transcriptome; differential gene expression; Cnaphalocrocis medinalis; glutathione S-transferases (GST); insecticide detoxification
• ISSN: 0739-4462; 1520-6327
• DOI: 10.1002/arch.21240

In insects, glutathione S‐transferases (GSTs) play critical roles in the detoxification of various insecticides, resulting in insecticide resistance. The rice leaffolder, Cnaphalocrocis medinalis, is an economically important pest of rice in Asia. GST genes have not been largely identified in this insect species. In the present study, by searching the transcriptome dataset, 25 candidate GST genes were identified in C. medinalis for the first time. Of these, 23 predicted GST proteins fell into five cytosolic classes (delta, epsilon, omega, sigma, and zeta), and two were assigned to the “unclassified” subgroup. Real‐time quantitative PCR analysis showed that these GST genes were differentially expressed in various tissues, including the midgut, Malpighian tubules, and fat body of larvae, and the antenna, abdomen, and leg of adults, indicating diversified functions for these genes. Transcription levels of CmGSTd2, CmGSTe6, and CmGSTe7 increased significantly in larvae following exposure to chlorpyrifos, suggesting that these GST genes could be involved in the detoxification of this insecticide. The results of our study pave the way to a better understanding of the detoxification system of C. medinalis.