Ca2+ activity signatures of myelin sheath formation and growth in vivo

During myelination, individual oligodendrocytes initially over-produce short myelin sheaths, which are either retracted or stabilized. By live-imaging oligodendrocyte Ca 2+ activity in vivo, we find that high-amplitude, long-duration Ca 2+ transients in sheaths prefigure retractions, mediated by cal...

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Bibliographic Details
Published inNature neuroscience Vol. 21; no. 1; pp. 19 - 23
Main Authors Baraban, Marion, Koudelka, Sigrid, Lyons, David A.
Format Journal Article
LanguageEnglish
Published New York Nature Publishing Group US 01.01.2018
Nature Publishing Group
Subjects
631/378/2571/219
631/378/2596/1705
Animal Genetics and Genomics
Axons
Behavioral Sciences
Biological Techniques
Biomedical and Life Sciences
Biomedicine
Brief Communication
Calcium imaging
Calcium signalling
Calpain
Elongation
Morphology
Myelin
Myelination
Neurobiology
Neurosciences
Oligodendrocytes
Sheaths
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Summary:During myelination, individual oligodendrocytes initially over-produce short myelin sheaths, which are either retracted or stabilized. By live-imaging oligodendrocyte Ca 2+ activity in vivo, we find that high-amplitude, long-duration Ca 2+ transients in sheaths prefigure retractions, mediated by calpain. Following stabilization, myelin sheaths grow along axons, and we find that higher-frequency Ca 2+ transient activity in sheaths precedes faster elongation. Our data implicate local Ca 2+ signaling in regulating distinct stages of myelination. The authors live-image zebrafish myelin sheath Ca 2+ activity in vivo and find that high-amplitude long-duration Ca 2+ transients precede calpain-dependent sheath retractions while frequent low-amplitude short-duration transients drive sheath growth.
ISSN:1097-6256
1546-1726
DOI:10.1038/s41593-017-0040-x