A new Mesorhizobium loti HAMBI 1129 phage isolated from polish soil

• Published in: Current microbiology Vol. 40; no. 5; pp. 341 - 343
• Main Authors: TURSKA-SZEWCZUK, A, RUSSA, R
• Format: Journal Article
• Language: English
• Published: New York, NY Springer 01.05.2000; Springer Nature B.V
• Subjects: Alphaproteobacteria - virology; Bacteriology; Bacteriophages - classification; Bacteriophages - isolation & purification; Bacteriophages - physiology; Biological and medical sciences; Burst size; Fundamental and applied biological sciences. Psychology; Legumes; Lotus corniculatus; Microbiology; Microscopy, Electron; Receptors, Virus - metabolism; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains; Rhizosphere; Soil Microbiology; Virology; Virus multiplication; Lotus corniculatus; Host virus relation; Virus; Characterization; Rhizosphere; Leguminosae; Dicotyledones; Angiospermae; Lipopolysaccharide; Bacteria; Isolate; Spermatophyta; Fodder crop; Rhizobiaceae; Phage; Biological receptor
• ISSN: 0343-8651; 1432-0991
• DOI: 10.1007/s002849910067

Phage A1 isolated from the rhizosphere of Lotus corniculatus was studied. It had a very narrow host range, as it was active only against Mesorhizobium loti HAMBI 1129. Phage A1 was classified as belonging to C Bradley's group bacteriophages. The latent period of A1 was 120-130 min and a burst size 13-17 particles per cell. The nature of the phage receptor was examined. Lipopolysaccharide from the phage-sensitive strain inactivated phage A1 in contrast to LPS from the phage-resistant bacteria. Purified LPS obtained from M. loti HAMBI 1129 had a high receptor activity with PhI(50) value of 0. 025 microgram/ml.