Sensitive Analysis of Protein Phosphatase Inhibitors by the Firefly Bioluminescence System: Application to PP1γ

Protein phosphatases are classified into types 1 (PP1) and 2 (PP2A, 2B, and 2C). We have already established a new analysis method for PP2A inhibitors by using the firefly bioluminescence system for detection. This method was successfully applied to determine the PP1γ inhibitory activity of known in...

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Bibliographic Details
Published inBioscience, biotechnology, and biochemistry Vol. 60; no. 8; pp. 1260 - 1264
Main Authors Sugiyama, Yukiteru, Fujimoto, Kazue, Ohtani, Ikuko I., Takai, Akira, Isobe, Minoru
Format Journal Article
LanguageEnglish
Published Tokyo Taylor & Francis 1996
Japan Society for Bioscience Biotechnology and Agrochemistry
Subjects
Analytical, structural and metabolic biochemistry
Biological and medical sciences
bioluminescence
Enzymes and enzyme inhibitors
Fundamental and applied biological sciences. Psychology
Hydrolases
PP1
PP1γ
protein phosphatase inhibitors
tautomycin
Catalytic subunit
Bioluminescence
Molecular form
Enzyme
Phosphoprotein phosphatase
Photinus-luciferin 4-monooxygenase (ATP-hydrolysing)
Phosphoric monoester hydrolases
Enzyme inhibitor
Esterases
Toxin
Enzymatic activity
Hydrolases
Oxidoreductases
Inhibition
Measurement method
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Summary:Protein phosphatases are classified into types 1 (PP1) and 2 (PP2A, 2B, and 2C). We have already established a new analysis method for PP2A inhibitors by using the firefly bioluminescence system for detection. This method was successfully applied to determine the PP1γ inhibitory activity of known inhibitors, i.e., calyculin A, microcystin-LR, and tautomycin, requiring less than 10 pmol of a sample.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.60.1260