Structure of bovine papillomavirus type 1 DNA in a transformed mouse cell line

• Published in: Journal of molecular biology Vol. 188; no. 1; pp. 1 - 13
• Main Authors: Allshire, Robin C., Bostock, Christopher J.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 05.03.1986; Elsevier
• Subjects: Animals; Autoradiography; Biological and medical sciences; Bovine papillomavirus; Bovine papillomavirus 1 - genetics; Bovine papillomavirus 1 - metabolism; Cell Line; Centrifugation, Density Gradient; DNA; DNA, Viral - metabolism; Electrophoresis, Agar Gel; Exonucleases - metabolism; Fundamental and applied biological sciences. Psychology; Genetics; genome; Mice; Microbiology; Mitosis; Nucleic Acid Hybridization; Papillomaviridae - genetics; Transformation, Genetic; Virology; Molecular structure; Transformed cell; Rodentia; Papovaviridae; Papillomavirus; Virus; Vertebrata; Mammalia; Cell line; Mouse; Oncogenic virus; Animal; DNA; Bovine papillomavirus 1
• ISSN: 0022-2836; 1089-8638
• DOI: 10.1016/0022-2836(86)90475-4

Linearized bovine papillomavirus type 1 (BPV-1) DNA was introduced into mouse C127 cells, where it recircularized and replicated as an intact monomeric, extrachromosomal circular form in the resulting transformants. These cells contained a mixture of complex high molecular weight forms that were converted to a linear form of approximately BPV-1 size upon digestion with an enzyme that cuts once within the BPV-1 genome. Further analysis of one of these cell lines revealed that these high molecular weight forms consisted of two components. One was detected on agarose gels as a diffuse smear of slow-migrating material representing linear forms that were tightly associated with host chromosomes, probably by integration. The second component was composed of discrete-sized oligomeric open and supercoiled extrachromosomal circular forms of up to approximately 48 × 10 3 base-pairs (6 tandemly linked BPV-1 genomes) in size. No catenated (interlocked) forms could be detected.