CD147 monoclonal antibody mediated by chitosan nanoparticles loaded with α-hederin enhances antineoplastic activity and cellular uptake in liver cancer cells

• Published in: Scientific reports Vol. 5; no. 1; p. 17904
• Main Authors: Zhu, Rong, Zhang, Chun-ge, Liu, Yang, Yuan, Zhi-qiang, Chen, Wei-liang, Yang, Shu-di, Li, Ji-zhao, Zhu, Wen-jing, Zhou, Xiao-feng, You, Ben-gang, Zhang, Xue-nong
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 07.12.2015; Nature Publishing Group
• Subjects: 631/67/1059/602; 692/308/153; Animals; Annexin A5 - metabolism; Antibodies, Monoclonal - pharmacology; Antineoplastic Agents - pharmacology; Apoptosis - drug effects; Basigin - immunology; Chitosan - chemistry; Endocytosis - drug effects; Flow Cytometry; Fluorescence; Hep G2 Cells; Humanities and Social Sciences; Humans; Imaging, Three-Dimensional; Intracellular Space - metabolism; Liver Neoplasms - pathology; Mice, Nude; Microscopy, Confocal; multidisciplinary; Nanoparticles - chemistry; Nanoparticles - ultrastructure; Oleanolic Acid - analogs & derivatives; Oleanolic Acid - chemical synthesis; Oleanolic Acid - chemistry; Oleanolic Acid - pharmacology; Particle Size; Propidium - metabolism; Saponins - chemical synthesis; Saponins - chemistry; Saponins - pharmacology; Science; Spectroscopy, Fourier Transform Infrared; Spectroscopy, Near-Infrared; Subcellular Fractions - metabolism
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep17904

An antibody that specifically interacts with an antigen could be applied to an active targeting delivery system. In this study, CD147 antibody was coupled with α-hed chitosan nanoparticles (α-Hed-CS-NPs). α-Hed-CS-CD147-NPs were round and spherical in shape, with an average particle size of 148.23 ± 1.75 nm. The half-maximum inhibiting concentration (IC50) of α-Hed-CS-CD147-NPs in human liver cancer cell lines HepG2 and SMMC-7721 was lower than that of free α-Hed and α-Hed-CS-NPs. α-Hed-induced cell death was mainly triggered by apoptosis. The increase in intracellular accumulation of α-Hed-CS-CD147-NPs was also related to CD147-mediated internalization through the Caveolae-dependent pathway and lysosomal escape. The higher targeting antitumor efficacy of α-Hed-CS-CD147-NPs than that α-Hed-CS-NPs was attributed to its stronger fluorescence intensity in the tumor site in nude mice.