Serum Amyloid A3 Fuels a Feed-Forward Inflammatory Response to the Bacterial Amyloid Curli in the Enteric Nervous System

• Published in: Cellular and molecular gastroenterology and hepatology Vol. 18; no. 1; pp. 89 - 104
• Main Authors: Verstraelen, Peter, Van Remoortel, Samuel, De Loose, Nouchin, Verboven, Rosanne, Garcia-Diaz Barriga, Gerardo, Christmann, Anne, Gries, Manuela, Bessho, Shingo, Li, Jing, Guerra, Carmen, Tükel, Çagla, Martinez, Sales Ibiza, Schäfer, Karl-Herbert, Timmermans, Jean-Pierre, De Vos, Winnok H.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.01.2024; Elsevier
• Subjects: Animals; Bacterial Proteins - metabolism; Colitis - immunology; Colitis - microbiology; Colitis - pathology; Curli; Cytokines - metabolism; Dual Leucine Zipper Kinase; Enteric Nervous System; Enteric Nervous System - immunology; Enteric Nervous System - metabolism; Enteric Nervous System - pathology; Gastrointestinal Microbiome - immunology; Inflammation - immunology; Inflammation - metabolism; Inflammation - pathology; Mice; Mice, Inbred C57BL; Mice, Knockout; Microbiome–Gut–Brain Axis; Neuroglia - immunology; Neuroglia - metabolism; Neuroglia - pathology; Neurons - metabolism; Neurons - pathology; Original Research; Serum Amyloid A Protein - genetics; Serum Amyloid A Protein - metabolism; Serum Amyloid A3; CXCL; DLK; Dual Leucine Zipper Kinase; Pen/Strep; JNK; SAA3; mRNA; PFA; ENS; FACS; Enteric Nervous System; LPS; DMEM; TBS-T; γH2AX; cDNA; qPCR; TLR; PBS; GI; AD; DSS; E14; CCL; SOX10; NA; EdU; Microbiome–Gut–Brain Axis; Curli; Serum Amyloid A3; ELISA; rSAA3
• ISSN: 2352-345X; 2352-345X
• DOI: 10.1016/j.jcmgh.2024.03.013

Mounting evidence suggests the gastrointestinal microbiome is a determinant of peripheral immunity and central neurodegeneration, but the local disease mechanisms remain unknown. Given its potential relevance for early diagnosis and therapeutic intervention, we set out to map the pathogenic changes induced by bacterial amyloids in the gastrointestinal tract and its enteric nervous system. To examine the early response, we challenged primary murine myenteric networks with curli, the prototypical bacterial amyloid, and performed shotgun RNA sequencing and multiplex enzyme-linked immunosorbent assay. Using enteric neurosphere-derived glial and neuronal cell cultures, as well as in vivo curli injections into the colon wall, we further scrutinized curli-induced pathogenic pathways. Curli induced a proinflammatory response, with strong up-regulation of Saa3 and the secretion of several cytokines. This proinflammatory state was induced primarily in enteric glia, was accompanied by increased levels of DNA damage and replication, and triggered the influx of immune cells in vivo. The addition of recombinant Serum Amyloid A3 (SAA3) was sufficient to recapitulate this specific proinflammatory phenotype while Saa3 knock-out attenuated curli-induced DNA damage and replication. Similar to curli, recombinant SAA3 caused a strong up-regulation of Saa3 transcripts, illustrating its self-amplifying potential . Since colonization of curli-producing Salmonella and dextran sulfate sodium–induced colitis triggered a significant increase in Saa3 transcripts as well, we assume SAA3plays a central role in enteric dysfunction. Inhibition of dual leucine zipper kinase, an upstream regulator of the c-Jun N-terminal kinase pathway responsible for SAA3 production, attenuated curli- and recombinant SAA3-induced Saa3 up-regulation, DNA damage, and replication in enteric glia. Our results position SAA3 as an important mediator of gastrointestinal vulnerability to bacterial-derived amyloids and demonstrate the potential of dual leucine zipper kinase inhibition to dampen enteric pathology. [Display omitted]