Novel loci associated with resistance to downy and powdery mildew in grapevine

Among the main challenges in current viticulture, there is the increasing demand for sustainability in the protection from fungal diseases, such as downy mildew (DM) and powdery mildew (PM). Breeding disease-resistant grapevine varieties is a key strategy for better managing fungicide inputs. This s...

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Published inFrontiers in plant science Vol. 15; p. 1386225
Main Authors Ricciardi, Valentina, Crespan, Manna, Maddalena, Giuliana, Migliaro, Daniele, Brancadoro, Lucio, Maghradze, David, Failla, Osvaldo, Toffolatti, Silvia Laura, De Lorenzis, Gabriella
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 2024
Subjects
GWAS
phenotyping
Ren
Rpv
SNP
SSR
GWAS
SSR
SNP
candidate genes
Rpv
Ren
phenotyping
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Summary:Among the main challenges in current viticulture, there is the increasing demand for sustainability in the protection from fungal diseases, such as downy mildew (DM) and powdery mildew (PM). Breeding disease-resistant grapevine varieties is a key strategy for better managing fungicide inputs. This study explores the diversity of grapevine germplasm (cultivated and wild) from Caucasus and neighboring areas to identify genotypes resistant to DM and PM, based on 13 Simple Sequence Repeat (SSR) loci and phenotypical (artificial pathogen inoculation) analysis, and to identify loci associated with DM and PM resistance, Genome-Wide Association Analysis (GWAS) on Single Nucleotide Polymorphism (SNP) profiles. SSR analysis revealed resistant alleles for 16 out of 88 genotypes. Phenotypic data identified seven DM and 31 PM resistant genotypes. GWAS identified two new loci associated with DM resistance, located on chromosome 15 and 16 (designated as and ), and two with PM resistance, located on chromosome 6 and 17 (designated as and ). The four novel loci identified genomic regions rich in genes related to biotic stress response, such as genes involved in pathogen recognition, signal transduction and resistance response. This study highlights potential candidate genes associated with resistance to DM and PM, providing valuable insights for breeding programs for resistant varieties. To optimize their utilization, further functional characterization studies are recommended.
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ISSN:1664-462X
1664-462X
DOI:10.3389/fpls.2024.1386225