NF-κB is involved in the LPS-mediated proliferation and apoptosis of MAC-T epithelial cells as part of the subacute ruminal acidosis response in cows

• Published in: Biotechnology letters Vol. 38; no. 11; pp. 1839 - 1849
• Main Authors: Fan, Wen-Jie, Li, He-Ping, Zhu, He-Shui, Sui, Shi-Ping, Chen, Pei-Ge, Deng, Yue, Sui, Tong-Ming, Wang, Yue-Ying
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.11.2016
• Subjects: Acidosis; Acidosis - genetics; Acidosis - metabolism; Acidosis - pathology; Animals; Apoptosis; Apoptosis - drug effects; Applied Microbiology; Attenuation; Biochemistry; Biomedical and Life Sciences; Biotechnology; Cattle; Cattle Diseases - genetics; Cattle Diseases - metabolism; Cattle Diseases - pathology; Cell Line; Cell Proliferation - drug effects; Dose-Response Relationship, Drug; Epithelial Cells - cytology; Epithelial Cells - drug effects; Epithelial Cells - metabolism; Female; Gene Expression Regulation - drug effects; Inhibition; Inhibitors; Life Sciences; Lipopolysaccharides - pharmacology; MAP Kinase Signaling System - drug effects; Microbiology; NF-kappa B - genetics; NF-kappa B - metabolism; Original Research Paper; Pathways; Pyrrolidines - pharmacology; Thiocarbamates - pharmacology; Toxicity; Lipopolysaccharide; MAC-T cells; NF-кB; Bovine ruminal acidosis; Proliferation; Apoptosis
• ISSN: 0141-5492; 1573-6776
• DOI: 10.1007/s10529-016-2178-0

Objectives To determine the effect of NF-κB on cell proliferation and apoptosis, we investigate the expression of inflammation and apoptosis-related factors in the bovine mammary epithelial cell line, MAC-T. Results MAC-T cells were cultured in vitro and MTT and LDH assays used to determine the effects of lipopolysaccharide (LPS) on proliferation and cytotoxicity respectively. RT-PCR and western blotting were used to evaluate the effect of LPS and NF-κB inhibition [pyrrolidine dithiocarbamate (PDTC) treatment] on the expression of inflammation and apoptosis-related factors. LPS significantly inhibited MAC-T cell proliferation in a dose- and time-dependent manner. Furthermore, LPS promoted apoptosis while the NF-кB inhibitor PDTC attenuated this effect. After LPS treatment, the NF-кB signaling pathway was activated, and the expression of inflammation and apoptosis-related factors increased. When PDTC blocked NF-кB signaling, the expression of inflammation and apoptosis-related factors were decreased in MAC-T cells. Conclusions LPS activates the TLR4/NF-κB signaling pathway, inhibits proliferation and promotes apoptosis in MAC-T cells. NF-кB inhibition attenuates MAC-T cell apoptosis and TLR4/NF-κB signaling pathway. NF-кB inhibitor alleviating MAC-T cell apoptosis is presumably modulated by NF-кB.