Biochemical and genetic analysis of the yeast proteome with a movable ORF collection
Functional analysis of the proteome is an essential part of genomic research. To facilitate different proteomic approaches, a MORF (moveable ORF) library of 5854 yeast expression plasmids was constructed, each expressing a sequence-verified ORF as a C-terminal ORF fusion protein, under regulated con...
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Published in | Genes & development Vol. 19; no. 23; pp. 2816 - 2826 |
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Main Authors | , , , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Cold Spring Harbor Laboratory Press
01.12.2005
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Subjects | |
Online Access | Get full text |
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Summary: | Functional analysis of the proteome is an essential part of genomic research. To facilitate different proteomic approaches, a MORF (moveable ORF) library of 5854 yeast expression plasmids was constructed, each expressing a sequence-verified ORF as a C-terminal ORF fusion protein, under regulated control. Analysis of 5573 MORFs demonstrates that nearly all verified ORFs are expressed, suggests the authenticity of 48 ORFs characterized as dubious, and implicates specific processes including cytoskeletal organization and transcriptional control in growth inhibition caused by overexpression. Global analysis of glycosylated proteins identifies 109 new confirmed N-linked and 345 candidate glycoproteins, nearly doubling the known yeast glycome. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 These authors contributed equally to this work. Supplemental material is available at http://www.genesdev.org. E-MAIL elizabeth_grayhack@urmc.rochester.edu ; FAX (585) 271-2683. E-MAIL michael.snyder@yale.edu ; FAX (203) 432-3597. Corresponding authors. Article and publication are at http://www.genesdev.org/cgi/doi/10.1101/gad.1362105. |
ISSN: | 0890-9369 1549-5477 |
DOI: | 10.1101/gad.1362105 |